Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

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Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring th...

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Bibliographic Details
Main Authors: Sheh Omar, Nur Alia, Yap, Wing Fen, Abdullah, Jaafar, Che Engku Chik, Che Engku Noramalina, Mahdi, Mohd Adzir
Format: Article
Language:English
Published: Elsevier 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72252/1/Development%20of%20an%20optical%20sensor%20based%20on%20surface%20plasmon%20resonance%20phenomenon%20for%20diagnosis%20of%20dengue%20virus%20E-protein.pdf
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Summary:Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface.

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