Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats
Probiotic Lactobacillus casei Shirota (LcS) is a potential decontaminating agent of aflatoxin B1 (AFB1). However, few studies have investigated the influence of diet, especially a high protein (HP) diet, on the binding of AFB1 by probiotics. This research was conducted to determine the effect of HP...
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| Format: | Article |
| Language: | English |
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Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72420/1/Effect%20of%20high%20protein%20diet%20and%20probiotic%20Lactobacillus%20casei%20Shirota%20supplementation%20in%20aflatoxin%20B1-induced%20rats.pdf |
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| author | Zainuddin, Nurul Adilah Liew, Winnie Pui Pui Sabran, Mohd Redzwan Ismail, Amin |
| author_facet | Zainuddin, Nurul Adilah Liew, Winnie Pui Pui Sabran, Mohd Redzwan Ismail, Amin |
| author_sort | Zainuddin, Nurul Adilah |
| collection | UPM |
| description | Probiotic Lactobacillus casei Shirota (LcS) is a potential decontaminating agent of aflatoxin B1 (AFB1). However, few studies have investigated the influence of diet, especially a high protein (HP) diet, on the binding of AFB1 by probiotics. This research was conducted to determine the effect of HP diet on the ability of LcS to bind AFB1 and reduce aflatoxin M1 (AFM1) in AFB1-induced rats. Sprague Dawley rats were randomly divided into three groups: A (HP only), B (HP + 108 CFU LcS + 25 μg AFB1/kg BW), and C (HP + 25 μg AFB1/kg BW). Levels of AST and ALP were higher in all groups but other liver function’s biomarkers were in the normal range, and the liver’s histology showed no structural changes. The urea level of rats in group B (10.02 ± 0.73 mmol/l) was significantly lower () than that of rats in group A (10.82 ± 0.26 mmol/l). The presence of carcinoma in the small intestine and colon was more obvious in group C than in group B. Moreover, rats in group B had significantly () lower AFM1 concentration (0.39 ± 0.01 ng/ml) than rats in group C (5.22 ± 0.28 ng/ml). Through these findings, LcS supplementation with HP diet alleviated the adverse effects of AFB1 by preventing AFB1 absorption in the small intestine and reducing urinary AFM1. |
| first_indexed | 2024-03-06T10:09:11Z |
| format | Article |
| id | upm.eprints-72420 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:09:11Z |
| publishDate | 2018 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | upm.eprints-724202020-06-04T06:43:44Z http://psasir.upm.edu.my/id/eprint/72420/ Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats Zainuddin, Nurul Adilah Liew, Winnie Pui Pui Sabran, Mohd Redzwan Ismail, Amin Probiotic Lactobacillus casei Shirota (LcS) is a potential decontaminating agent of aflatoxin B1 (AFB1). However, few studies have investigated the influence of diet, especially a high protein (HP) diet, on the binding of AFB1 by probiotics. This research was conducted to determine the effect of HP diet on the ability of LcS to bind AFB1 and reduce aflatoxin M1 (AFM1) in AFB1-induced rats. Sprague Dawley rats were randomly divided into three groups: A (HP only), B (HP + 108 CFU LcS + 25 μg AFB1/kg BW), and C (HP + 25 μg AFB1/kg BW). Levels of AST and ALP were higher in all groups but other liver function’s biomarkers were in the normal range, and the liver’s histology showed no structural changes. The urea level of rats in group B (10.02 ± 0.73 mmol/l) was significantly lower () than that of rats in group A (10.82 ± 0.26 mmol/l). The presence of carcinoma in the small intestine and colon was more obvious in group C than in group B. Moreover, rats in group B had significantly () lower AFM1 concentration (0.39 ± 0.01 ng/ml) than rats in group C (5.22 ± 0.28 ng/ml). Through these findings, LcS supplementation with HP diet alleviated the adverse effects of AFB1 by preventing AFB1 absorption in the small intestine and reducing urinary AFM1. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72420/1/Effect%20of%20high%20protein%20diet%20and%20probiotic%20Lactobacillus%20casei%20Shirota%20supplementation%20in%20aflatoxin%20B1-induced%20rats.pdf Zainuddin, Nurul Adilah and Liew, Winnie Pui Pui and Sabran, Mohd Redzwan and Ismail, Amin (2018) Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats. Biomed Research International, 2018. art. no. 9568351. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2018/9568351/ 10.1155/2018/9568351 |
| spellingShingle | Zainuddin, Nurul Adilah Liew, Winnie Pui Pui Sabran, Mohd Redzwan Ismail, Amin Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title | Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title_full | Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title_fullStr | Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title_full_unstemmed | Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title_short | Effect of high protein diet and probiotic Lactobacillus casei Shirota supplementation in aflatoxin B1-induced rats |
| title_sort | effect of high protein diet and probiotic lactobacillus casei shirota supplementation in aflatoxin b1 induced rats |
| url | http://psasir.upm.edu.my/id/eprint/72420/1/Effect%20of%20high%20protein%20diet%20and%20probiotic%20Lactobacillus%20casei%20Shirota%20supplementation%20in%20aflatoxin%20B1-induced%20rats.pdf |
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