Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology
Clenbuterol (CLB) is an illegal antibiotic for livestock, which is misused as a growth promoter drug. In this study, an immunosensor modified with poly(3,4-ethylenedioxythiophene) (PEDOT), multi-walled carbon nanotubes (MWCNT) and anti-clenbuterol antibody (Ab) was developed for the detection of CLB...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Royal Society of Chemistry
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/72584/1/Enhancing%20clenbuterol%20immunosensor%20.pdf |
| _version_ | 1825950111586320384 |
|---|---|
| author | A. Talib, Nurul Ain Salam, Faridah Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang Azid, Mohd Zulkhairi Mirad, Razali Sulaiman, Yusran |
| author_facet | A. Talib, Nurul Ain Salam, Faridah Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang Azid, Mohd Zulkhairi Mirad, Razali Sulaiman, Yusran |
| author_sort | A. Talib, Nurul Ain |
| collection | UPM |
| description | Clenbuterol (CLB) is an illegal antibiotic for livestock, which is misused as a growth promoter drug. In this study, an immunosensor modified with poly(3,4-ethylenedioxythiophene) (PEDOT), multi-walled carbon nanotubes (MWCNT) and anti-clenbuterol antibody (Ab) was developed for the detection of CLB. A screen-printed carbon electrode (SPCE) was modified with PEDOT/MWCNT as a sensor platform before immobilizing Ab for specific CLB binding through a competitive-type immunoassay. Free CLB in the sample solution competed with clenbuterol-horseradish peroxide (CLB–HRP) to bind with Ab. A high current signal was obtained after optimization of the electrochemical immunoassay conditions (pH, incubation temperature, antigen (Ag) incubation time and % blocking) using the response surface methodology/central composite design (RSM/CCD). The developed immunosensor is highly reproducible and sensitive with good storage stability, which are necessary for practical application. In real sample application, this immunosensor produces comparable results with liquid chromatography-mass spectrometry; thus, it is useful for CLB screening and monitoring in real meat samples. |
| first_indexed | 2024-03-06T10:09:37Z |
| format | Article |
| id | upm.eprints-72584 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:09:37Z |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | upm.eprints-725842020-11-05T03:43:19Z http://psasir.upm.edu.my/id/eprint/72584/ Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology A. Talib, Nurul Ain Salam, Faridah Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang Azid, Mohd Zulkhairi Mirad, Razali Sulaiman, Yusran Clenbuterol (CLB) is an illegal antibiotic for livestock, which is misused as a growth promoter drug. In this study, an immunosensor modified with poly(3,4-ethylenedioxythiophene) (PEDOT), multi-walled carbon nanotubes (MWCNT) and anti-clenbuterol antibody (Ab) was developed for the detection of CLB. A screen-printed carbon electrode (SPCE) was modified with PEDOT/MWCNT as a sensor platform before immobilizing Ab for specific CLB binding through a competitive-type immunoassay. Free CLB in the sample solution competed with clenbuterol-horseradish peroxide (CLB–HRP) to bind with Ab. A high current signal was obtained after optimization of the electrochemical immunoassay conditions (pH, incubation temperature, antigen (Ag) incubation time and % blocking) using the response surface methodology/central composite design (RSM/CCD). The developed immunosensor is highly reproducible and sensitive with good storage stability, which are necessary for practical application. In real sample application, this immunosensor produces comparable results with liquid chromatography-mass spectrometry; thus, it is useful for CLB screening and monitoring in real meat samples. Royal Society of Chemistry 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72584/1/Enhancing%20clenbuterol%20immunosensor%20.pdf A. Talib, Nurul Ain and Salam, Faridah and Yusof, Nor Azah and Ahmad, Shahrul Ainliah Alang and Azid, Mohd Zulkhairi and Mirad, Razali and Sulaiman, Yusran (2018) Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology. RSC Advances, 8. pp. 1-11. ISSN 2046-2069 10.1039/c8ra00109j |
| spellingShingle | A. Talib, Nurul Ain Salam, Faridah Yusof, Nor Azah Ahmad, Shahrul Ainliah Alang Azid, Mohd Zulkhairi Mirad, Razali Sulaiman, Yusran Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title | Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title_full | Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title_fullStr | Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title_full_unstemmed | Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title_short | Enhancing clenbuterol immunosensor based on poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube performance by response surface methodology |
| title_sort | enhancing clenbuterol immunosensor based on poly 3 4 ethylenedioxythiophene multi walled carbon nanotube performance by response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/72584/1/Enhancing%20clenbuterol%20immunosensor%20.pdf |
| work_keys_str_mv | AT atalibnurulain enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT salamfaridah enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT yusofnorazah enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT ahmadshahrulainliahalang enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT azidmohdzulkhairi enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT miradrazali enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology AT sulaimanyusran enhancingclenbuterolimmunosensorbasedonpoly34ethylenedioxythiophenemultiwalledcarbonnanotubeperformancebyresponsesurfacemethodology |