Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein
Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield t...
| Main Authors: | , , , |
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| פורמט: | Article |
| שפה: | English |
| יצא לאור: |
Elsevier
2018
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| גישה מקוונת: | http://psasir.upm.edu.my/id/eprint/72609/1/Escherichia%20coli%20tolerance%20.pdf |
| _version_ | 1825950114707931136 |
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| author | Huwaidi, Alaa Hassan Ibrahim Ahmed Pathak, Nishit Amir Hamzah, Amir Syahir Ikeno, Shinya |
| author_facet | Huwaidi, Alaa Hassan Ibrahim Ahmed Pathak, Nishit Amir Hamzah, Amir Syahir Ikeno, Shinya |
| author_sort | Huwaidi, Alaa Hassan Ibrahim Ahmed |
| collection | UPM |
| description | Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide showed a higher number of colony-forming units per dilution under UVA and UVC treatment. These results suggested that expression of the short peptide could be useful for the development of genetically modified organisms and in applications that require resilience of organisms to UV radiation. |
| first_indexed | 2024-03-06T10:09:40Z |
| format | Article |
| id | upm.eprints-72609 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:09:40Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-726092022-08-01T08:52:03Z http://psasir.upm.edu.my/id/eprint/72609/ Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein Huwaidi, Alaa Hassan Ibrahim Ahmed Pathak, Nishit Amir Hamzah, Amir Syahir Ikeno, Shinya Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide showed a higher number of colony-forming units per dilution under UVA and UVC treatment. These results suggested that expression of the short peptide could be useful for the development of genetically modified organisms and in applications that require resilience of organisms to UV radiation. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72609/1/Escherichia%20coli%20tolerance%20.pdf Huwaidi, Alaa Hassan Ibrahim Ahmed and Pathak, Nishit and Amir Hamzah, Amir Syahir and Ikeno, Shinya (2018) Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein. Biochemical and Biophysical Research Communications, 503 (2). 910 - 914. ISSN 0006-291X; ESSN: 1090-2104 https://www.sciencedirect.com/science/article/pii/S0006291X18314074?via%3Dihub 10.1016/j.bbrc.2018.06.095 |
| spellingShingle | Huwaidi, Alaa Hassan Ibrahim Ahmed Pathak, Nishit Amir Hamzah, Amir Syahir Ikeno, Shinya Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title | Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title_full | Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title_fullStr | Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title_full_unstemmed | Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title_short | Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| title_sort | escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein |
| url | http://psasir.upm.edu.my/id/eprint/72609/1/Escherichia%20coli%20tolerance%20.pdf |
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