Evaluation of expression of OsAMT1;1 under different nitrogen concentration
The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Effi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The Malaysian Society For Biochemistry & Molecular Biology
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf |
| _version_ | 1825950118858194944 |
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| author | Mohd Amin, Adibah Abdullah, Siti Nor Akmar Musa, Mohamed Hanafi |
| author_facet | Mohd Amin, Adibah Abdullah, Siti Nor Akmar Musa, Mohamed Hanafi |
| author_sort | Mohd Amin, Adibah |
| collection | UPM |
| description | The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Efficiency (NUE) at the molecular level. The objective of this studies is to evaluate OsAMT1;1 expression on landraces with different NUE under different nitrogen level. To further understand the molecular basis of responsiveness of these two contrasting upland rice landraces to different N inputs at the N uptake level, a laboratory experiment was conducted to study how members of ammonium transporter gene family respond to different nitrogen conditions. Two upland varieties, Landrace III (high Nitrogen Use Efficiency) and Landrace I (low Nitrogen Use Efficiency) were grown in 4 nitrogen levels (0.05mM NH4NO3, 0.1mM NH4NO3, 1mM NH4NO3 and 2mM NH4NO3). Quantitative RT-PCR was used to study the expression of patterns of rice OsAMT1;1 genes. Results from this study showed that Landrace III has relatively higher expression of OsAMT1;1 compared to Landrace I for all treatments. Expression of OsAMT1;1 is clearly up-regulated in response to low-nitrogen treatment. Expression levels of OsAMT1;1 in high-NUE upland rice landrace was higher compared to low-NUE upland rice landrace. These results suggested, expression of OsAMT1;1 had certain degree of influenced in NUE of upland rice landraces. |
| first_indexed | 2024-03-06T10:09:44Z |
| format | Article |
| id | upm.eprints-72633 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:09:44Z |
| publishDate | 2018 |
| publisher | The Malaysian Society For Biochemistry & Molecular Biology |
| record_format | dspace |
| spelling | upm.eprints-726332020-11-17T05:43:21Z http://psasir.upm.edu.my/id/eprint/72633/ Evaluation of expression of OsAMT1;1 under different nitrogen concentration Mohd Amin, Adibah Abdullah, Siti Nor Akmar Musa, Mohamed Hanafi The NH4+ and NO3− are N sources available for plant nutrition in most soils. The NH4+ role in plant nutrition has most likely been underestimated. This study provides basic information on the expression of OsAMT1;1 that is needed in understanding factors contributing to upland rice Nitrogen Use Efficiency (NUE) at the molecular level. The objective of this studies is to evaluate OsAMT1;1 expression on landraces with different NUE under different nitrogen level. To further understand the molecular basis of responsiveness of these two contrasting upland rice landraces to different N inputs at the N uptake level, a laboratory experiment was conducted to study how members of ammonium transporter gene family respond to different nitrogen conditions. Two upland varieties, Landrace III (high Nitrogen Use Efficiency) and Landrace I (low Nitrogen Use Efficiency) were grown in 4 nitrogen levels (0.05mM NH4NO3, 0.1mM NH4NO3, 1mM NH4NO3 and 2mM NH4NO3). Quantitative RT-PCR was used to study the expression of patterns of rice OsAMT1;1 genes. Results from this study showed that Landrace III has relatively higher expression of OsAMT1;1 compared to Landrace I for all treatments. Expression of OsAMT1;1 is clearly up-regulated in response to low-nitrogen treatment. Expression levels of OsAMT1;1 in high-NUE upland rice landrace was higher compared to low-NUE upland rice landrace. These results suggested, expression of OsAMT1;1 had certain degree of influenced in NUE of upland rice landraces. The Malaysian Society For Biochemistry & Molecular Biology 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf Mohd Amin, Adibah and Abdullah, Siti Nor Akmar and Musa, Mohamed Hanafi (2018) Evaluation of expression of OsAMT1;1 under different nitrogen concentration. Malaysian Journal of Biochemistry and Molecular Biology, 21 (2). pp. 1-5. ISSN 2600-9005 https://msbmb2010.wixsite.com/mjbmb/copy-of-home-3 |
| spellingShingle | Mohd Amin, Adibah Abdullah, Siti Nor Akmar Musa, Mohamed Hanafi Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title | Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title_full | Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title_fullStr | Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title_full_unstemmed | Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title_short | Evaluation of expression of OsAMT1;1 under different nitrogen concentration |
| title_sort | evaluation of expression of osamt1 1 under different nitrogen concentration |
| url | http://psasir.upm.edu.my/id/eprint/72633/1/Evaluation%20of%20expression%20.pdf |
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