Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system
This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Verlag
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72695/1/Extractive%20purification%20of%20recombinant%20thermostable%20lipase%20from%20fermentation%20broth%20of%20Escherichia%20coli%20using%20an%20aqueous%20polyethylene%20glycol%20impregnated%20resin%20system.pdf |
| _version_ | 1825950129108025344 |
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| author | Tan, Joo Shun Abbasiliasi, Sahar Ariff, Arbakariya Ng, Hui Suan Abu Bakar, Mohamad Hafizi Chow, Yin Hui |
| author_facet | Tan, Joo Shun Abbasiliasi, Sahar Ariff, Arbakariya Ng, Hui Suan Abu Bakar, Mohamad Hafizi Chow, Yin Hui |
| author_sort | Tan, Joo Shun |
| collection | UPM |
| description | This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9. |
| first_indexed | 2024-03-06T10:09:53Z |
| format | Article |
| id | upm.eprints-72695 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:09:53Z |
| publishDate | 2018 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | upm.eprints-726952021-01-05T14:05:16Z http://psasir.upm.edu.my/id/eprint/72695/ Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system Tan, Joo Shun Abbasiliasi, Sahar Ariff, Arbakariya Ng, Hui Suan Abu Bakar, Mohamad Hafizi Chow, Yin Hui This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9. Springer Verlag 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72695/1/Extractive%20purification%20of%20recombinant%20thermostable%20lipase%20from%20fermentation%20broth%20of%20Escherichia%20coli%20using%20an%20aqueous%20polyethylene%20glycol%20impregnated%20resin%20system.pdf Tan, Joo Shun and Abbasiliasi, Sahar and Ariff, Arbakariya and Ng, Hui Suan and Abu Bakar, Mohamad Hafizi and Chow, Yin Hui (2018) Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system. 3 Biotech, 8 (6). pp. 1-7. ISSN 2190-572X; ESSN: 2190-5738 https://pubmed.ncbi.nlm.nih.gov/29938157/ 10.1007/s13205-018-1295-y |
| spellingShingle | Tan, Joo Shun Abbasiliasi, Sahar Ariff, Arbakariya Ng, Hui Suan Abu Bakar, Mohamad Hafizi Chow, Yin Hui Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title | Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title_full | Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title_fullStr | Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title_full_unstemmed | Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title_short | Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| title_sort | extractive purification of recombinant thermostable lipase from fermentation broth of escherichia coli using an aqueous polyethylene glycol impregnated resin system |
| url | http://psasir.upm.edu.my/id/eprint/72695/1/Extractive%20purification%20of%20recombinant%20thermostable%20lipase%20from%20fermentation%20broth%20of%20Escherichia%20coli%20using%20an%20aqueous%20polyethylene%20glycol%20impregnated%20resin%20system.pdf |
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