First molecular detection of porcine bocavirus in Malaysia
Several strains of porcine bocaviruses have been reported worldwide since their first detection in Sweden in 2009. Subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. Although Malaysia is host to a self-sufficient swine li...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72762/1/First%20molecular%20detection%20of%20porcine%20bocavirus%20in%20Malaysia%20.pdf |
| _version_ | 1825950139254046720 |
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| author | Jacob, Daniel Mohan Lee, Chee Yien Arshad, Siti Suri Selvarajah, Gayathri Thevi Bande, Faruku Ong, Bee Lee Ooi, Peck Toung |
| author_facet | Jacob, Daniel Mohan Lee, Chee Yien Arshad, Siti Suri Selvarajah, Gayathri Thevi Bande, Faruku Ong, Bee Lee Ooi, Peck Toung |
| author_sort | Jacob, Daniel Mohan |
| collection | UPM |
| description | Several strains of porcine bocaviruses have been reported worldwide since their first detection in Sweden in 2009. Subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. Although Malaysia is host to a self-sufficient swine livestock industry, there is no study that describes porcine bocavirus in the country. This report is the first to describe porcine bocavirus (PBoV) in Malaysian swine herds. PBoV was identified in various tissues from sick and runt pigs using the conventional PCR method with primers targeting conserved regions encoding for the nonstructural protein (NS1) gene. Out of 103 samples tested from 17 pigs, 32 samples from 15 pigs were positive for porcine bocavirus. In addition, a higher detection rate was identified from mesenteric lymph nodes (52.9%), followed by tonsil (37.0%), and lungs (33.3%). Pairwise comparison and phylogenetic analyses based on a 658-bp fragment of NS1 gene revealed that the Malaysian PBoV strains are highly similar to PBoV3 isolated in Minnesota, USA. The presence of porcine bocavirus in Malaysia and their phylogenetic bond was marked for the first time by this study. Further studies will establish the molecular epidemiology of PBoV in Malaysia and clarify pathogenicity of the local isolates. |
| first_indexed | 2024-03-06T10:10:03Z |
| format | Article |
| id | upm.eprints-72762 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:10:03Z |
| publishDate | 2018 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-727622021-02-02T03:04:56Z http://psasir.upm.edu.my/id/eprint/72762/ First molecular detection of porcine bocavirus in Malaysia Jacob, Daniel Mohan Lee, Chee Yien Arshad, Siti Suri Selvarajah, Gayathri Thevi Bande, Faruku Ong, Bee Lee Ooi, Peck Toung Several strains of porcine bocaviruses have been reported worldwide since their first detection in Sweden in 2009. Subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. Although Malaysia is host to a self-sufficient swine livestock industry, there is no study that describes porcine bocavirus in the country. This report is the first to describe porcine bocavirus (PBoV) in Malaysian swine herds. PBoV was identified in various tissues from sick and runt pigs using the conventional PCR method with primers targeting conserved regions encoding for the nonstructural protein (NS1) gene. Out of 103 samples tested from 17 pigs, 32 samples from 15 pigs were positive for porcine bocavirus. In addition, a higher detection rate was identified from mesenteric lymph nodes (52.9%), followed by tonsil (37.0%), and lungs (33.3%). Pairwise comparison and phylogenetic analyses based on a 658-bp fragment of NS1 gene revealed that the Malaysian PBoV strains are highly similar to PBoV3 isolated in Minnesota, USA. The presence of porcine bocavirus in Malaysia and their phylogenetic bond was marked for the first time by this study. Further studies will establish the molecular epidemiology of PBoV in Malaysia and clarify pathogenicity of the local isolates. Springer 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72762/1/First%20molecular%20detection%20of%20porcine%20bocavirus%20in%20Malaysia%20.pdf Jacob, Daniel Mohan and Lee, Chee Yien and Arshad, Siti Suri and Selvarajah, Gayathri Thevi and Bande, Faruku and Ong, Bee Lee and Ooi, Peck Toung (2018) First molecular detection of porcine bocavirus in Malaysia. Tropical Animal Health and Production, 50 (4). 733 - 739. ISSN 0049-4747; ESSN: 1573-7438 https://link.springer.com/article/10.1007/s11250-017-1489-z 10.1007/s11250-017-1489-z |
| spellingShingle | Jacob, Daniel Mohan Lee, Chee Yien Arshad, Siti Suri Selvarajah, Gayathri Thevi Bande, Faruku Ong, Bee Lee Ooi, Peck Toung First molecular detection of porcine bocavirus in Malaysia |
| title | First molecular detection of porcine bocavirus in Malaysia |
| title_full | First molecular detection of porcine bocavirus in Malaysia |
| title_fullStr | First molecular detection of porcine bocavirus in Malaysia |
| title_full_unstemmed | First molecular detection of porcine bocavirus in Malaysia |
| title_short | First molecular detection of porcine bocavirus in Malaysia |
| title_sort | first molecular detection of porcine bocavirus in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/72762/1/First%20molecular%20detection%20of%20porcine%20bocavirus%20in%20Malaysia%20.pdf |
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