From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha
Mikania micrantha is commonly consumed as traditional medicine in some countries, including Malaysia. Little is known about the antioxidant properties and phytochemicals of M. micrantha. This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxida...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Smith and Franklin Academic Publishing
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72796/1/From%20weed%20to%20medicinal%20plant.pdf |
| _version_ | 1825950144322863104 |
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| author | Ishak, Amirah Haziyah Shafie, Nurul Husna Mohd Esa, Norhaizan Bahari, Hasnah Ismail, Amin |
| author_facet | Ishak, Amirah Haziyah Shafie, Nurul Husna Mohd Esa, Norhaizan Bahari, Hasnah Ismail, Amin |
| author_sort | Ishak, Amirah Haziyah |
| collection | UPM |
| description | Mikania micrantha is commonly consumed as traditional medicine in some countries, including Malaysia. Little is known about the antioxidant properties and phytochemicals of M. micrantha. This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacities of the leaves and stems of M. micrantha of hot water, cold water, 70% ethanol, ethyl acetate, and hexane extracts. Folin-Ciocalteu and aluminium chloride colorimetric assays were used to determine the TPC and TFC, respectively. The antioxidant capacities were determined using rapid, inexpensive and small-scale microplate of five different antioxidant assays. Gas chromatography-mass spectrometry (GC-MS) was used to chemically profile and characterize the phytochemicals. In comparison with different solvents, the ethyl acetate stems (EAS) and leaves (EAL) extracts of M. micrantha had the significantly greatest TPC (141±0.51 mg gallic acid equivalent/g) and TFC (70.1±0.92 catechin equivalent/g), respectively. Moreover, EAS extract had the significantly greatest antioxidant capacities using DPPH (EC50=324±61.4 μg/mL), ABTS (0.53±0.01 mmol trolox equivalent/g), FRAP (1.28±0.05 mmol Fe2+/g), phosphomolybdenum antioxidative power (219±7.03 mg ascorbic acid equivalent/g), and β-carotene bleaching (108±2.23%) assays. GC-MS analysis of EAS showed the presence of sesquiterpenes (30.46%), phenol (16.38%), and alkane hydrocarbons (10.45%), which may contribute to its antioxidant capacities. These findings suggest the stems extract of M. micrantha using ethyl acetate as the potential source of natural antioxidant agents and its utilization to prevent oxidative damage-related diseases could be further explored. |
| first_indexed | 2024-03-06T10:10:07Z |
| format | Article |
| id | upm.eprints-72796 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:10:07Z |
| publishDate | 2018 |
| publisher | Smith and Franklin Academic Publishing |
| record_format | dspace |
| spelling | upm.eprints-727962021-02-08T00:47:07Z http://psasir.upm.edu.my/id/eprint/72796/ From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha Ishak, Amirah Haziyah Shafie, Nurul Husna Mohd Esa, Norhaizan Bahari, Hasnah Ismail, Amin Mikania micrantha is commonly consumed as traditional medicine in some countries, including Malaysia. Little is known about the antioxidant properties and phytochemicals of M. micrantha. This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacities of the leaves and stems of M. micrantha of hot water, cold water, 70% ethanol, ethyl acetate, and hexane extracts. Folin-Ciocalteu and aluminium chloride colorimetric assays were used to determine the TPC and TFC, respectively. The antioxidant capacities were determined using rapid, inexpensive and small-scale microplate of five different antioxidant assays. Gas chromatography-mass spectrometry (GC-MS) was used to chemically profile and characterize the phytochemicals. In comparison with different solvents, the ethyl acetate stems (EAS) and leaves (EAL) extracts of M. micrantha had the significantly greatest TPC (141±0.51 mg gallic acid equivalent/g) and TFC (70.1±0.92 catechin equivalent/g), respectively. Moreover, EAS extract had the significantly greatest antioxidant capacities using DPPH (EC50=324±61.4 μg/mL), ABTS (0.53±0.01 mmol trolox equivalent/g), FRAP (1.28±0.05 mmol Fe2+/g), phosphomolybdenum antioxidative power (219±7.03 mg ascorbic acid equivalent/g), and β-carotene bleaching (108±2.23%) assays. GC-MS analysis of EAS showed the presence of sesquiterpenes (30.46%), phenol (16.38%), and alkane hydrocarbons (10.45%), which may contribute to its antioxidant capacities. These findings suggest the stems extract of M. micrantha using ethyl acetate as the potential source of natural antioxidant agents and its utilization to prevent oxidative damage-related diseases could be further explored. Smith and Franklin Academic Publishing 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72796/1/From%20weed%20to%20medicinal%20plant.pdf Ishak, Amirah Haziyah and Shafie, Nurul Husna and Mohd Esa, Norhaizan and Bahari, Hasnah and Ismail, Amin (2018) From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha. International Journal of Agriculture and Biology, 20 (3). 561 - 568. ISSN 1560-8530; ESSN: 1814-9596 https://www.cabdirect.org/globalhealth/abstract/20193099691 10.17957/IJAB/15.0522 |
| spellingShingle | Ishak, Amirah Haziyah Shafie, Nurul Husna Mohd Esa, Norhaizan Bahari, Hasnah Ismail, Amin From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title | From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title_full | From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title_fullStr | From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title_full_unstemmed | From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title_short | From weed to medicinal plant: antioxidant capacities and phytochemicals of various extracts of Mikania micrantha |
| title_sort | from weed to medicinal plant antioxidant capacities and phytochemicals of various extracts of mikania micrantha |
| url | http://psasir.upm.edu.my/id/eprint/72796/1/From%20weed%20to%20medicinal%20plant.pdf |
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