Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate
Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf |
| _version_ | 1796967290717077504 |
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| author | Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming |
| author_facet | Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming |
| author_sort | Lo, Seong Koon |
| collection | UPM |
| description | Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as well as the effect of molecular sieves as a water adsorbent were investigated. Rhizomucor miehei lipase (Lipozyme RM IM) was found to be most effective among the lipases screened. The following conditions yielded 70.0% (w/w) DAG: 5 h reaction time, 65°C reaction temperature, 10% (w/w) Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% (w/w) molecular sieves. DAG synthesis of 12.4% (w/w) was still observed at 10% (w/w) water content. 84.2% (w/w) of DAG was obtained after purification. The DAG oil comprised predominantly of 1-oleoyl-3-linoleoyl-glycerol (28.5%), 1,3-diolein (22.7%), 1-oleoyl-2-linoleoyl-glycerol (17.9%), and 1,2-diolein (10.9%). Fatty acid profile was similar to that of refined, bleached and deodorised (RBD) corn oil. The ratio of 1,3- to 1,2-positional isomers of DAG was at 1.82:1. |
| first_indexed | 2024-03-06T07:11:46Z |
| format | Article |
| id | upm.eprints-7280 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:46Z |
| publishDate | 2005 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-72802015-10-06T00:25:43Z http://psasir.upm.edu.my/id/eprint/7280/ Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming Diacylglycerols (DAG) were enzymatically synthesized by lipase-catalyzed esterification of glycerol with fatty acids from corn oil deodorizer distillate (CrODD). Effects of reaction parameters such as reaction time, temperature, enzyme type, enzyme load, substrate mole ratio, and water content, as well as the effect of molecular sieves as a water adsorbent were investigated. Rhizomucor miehei lipase (Lipozyme RM IM) was found to be most effective among the lipases screened. The following conditions yielded 70.0% (w/w) DAG: 5 h reaction time, 65°C reaction temperature, 10% (w/w) Lipozyme RM IM, 2.5:1 fatty acid to glycerol molar ratio, and 30% (w/w) molecular sieves. DAG synthesis of 12.4% (w/w) was still observed at 10% (w/w) water content. 84.2% (w/w) of DAG was obtained after purification. The DAG oil comprised predominantly of 1-oleoyl-3-linoleoyl-glycerol (28.5%), 1,3-diolein (22.7%), 1-oleoyl-2-linoleoyl-glycerol (17.9%), and 1,2-diolein (10.9%). Fatty acid profile was similar to that of refined, bleached and deodorised (RBD) corn oil. The ratio of 1,3- to 1,2-positional isomers of DAG was at 1.82:1. Taylor & Francis 2005-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf Lo, Seong Koon and Baharin, Badlishah Sham and Tan, Chin Ping and Lai, Oi Ming (2005) Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate. Food Biotechnology, 18 (3). pp. 265-278. ISSN 0890-5436 http://dx.doi.org/10.1081/FBT-200035014 10.1081/FBT-200035014 English |
| spellingShingle | Lo, Seong Koon Baharin, Badlishah Sham Tan, Chin Ping Lai, Oi Ming Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title | Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title_full | Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title_fullStr | Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title_full_unstemmed | Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title_short | Enzyme-Catalyzed Production and Chemical Composition of Diacylglycerols from Corn Oil Deodorizer Distillate |
| title_sort | enzyme catalyzed production and chemical composition of diacylglycerols from corn oil deodorizer distillate |
| url | http://psasir.upm.edu.my/id/eprint/7280/1/Enzyme.pdf |
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