Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors
Boesenbergia rotunda, traditionally used to relieve stomach, abdomen, joint, muscle, and rheumatic pain was also reported for its antinociceptive effect on a mouse model. However, the possible pain relief effect of Boesenbergia rotunda ethanolic extract (BREE) via the inhibition to the neural pain p...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Biome Journals
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/73296/1/ROTUNDA.pdf |
| _version_ | 1825950181318721536 |
|---|---|
| author | Perimal, Enoch Kumar Chia, Jasmine Siew Min Gopalsamy, Banulata Hussain, Mohd Khairi Omar Farouk, Ahmad Akira Sulaiman, Mohd Roslan Amir Ramadan, Ammar Izzati Wong, Siong Jun Ghazali, Farihah Hanani |
| author_facet | Perimal, Enoch Kumar Chia, Jasmine Siew Min Gopalsamy, Banulata Hussain, Mohd Khairi Omar Farouk, Ahmad Akira Sulaiman, Mohd Roslan Amir Ramadan, Ammar Izzati Wong, Siong Jun Ghazali, Farihah Hanani |
| author_sort | Perimal, Enoch Kumar |
| collection | UPM |
| description | Boesenbergia rotunda, traditionally used to relieve stomach, abdomen, joint, muscle, and rheumatic pain was also reported for its antinociceptive effect on a mouse model. However, the possible pain relief effect of Boesenbergia rotunda ethanolic extract (BREE) via the inhibition to the neural pain pathway remains to be elucidated. This study investigated the inhibitory effect of BREE on compound action potentials (CAPs) and the possible involvement of the opioid receptors. The changes in the CAPs amplitudes of the frog’s sciatic nerves were evaluated following the exposure to three different dosages of BREE (1, 3 and 10 mg/ml and morphine (3 mg/ml). In another set of experiment, the nerves were pretreated with a non-selective opioid receptor antagonist, naloxone (0.1 mg/ml), before exposing the nerve to BREE (1 mg/ml) to investigate the involvement of opioid receptors in the CAPs inhibitory mechanism. The outcome showed a reduction in the CAPs amplitudes when treated with BREE (1, 3 and 10 mg/ml) whereby the effect was reversible. The CAPs inhibition by BREE was absent when the opioid receptors were blocked. Taken together, these findings suggest that BREE-induced CAPs amplitude reduction involves the activation of opioid receptors. |
| first_indexed | 2024-03-06T10:10:41Z |
| format | Article |
| id | upm.eprints-73296 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:10:41Z |
| publishDate | 2018 |
| publisher | Biome Journals |
| record_format | dspace |
| spelling | upm.eprints-732962020-11-30T08:39:02Z http://psasir.upm.edu.my/id/eprint/73296/ Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors Perimal, Enoch Kumar Chia, Jasmine Siew Min Gopalsamy, Banulata Hussain, Mohd Khairi Omar Farouk, Ahmad Akira Sulaiman, Mohd Roslan Amir Ramadan, Ammar Izzati Wong, Siong Jun Ghazali, Farihah Hanani Boesenbergia rotunda, traditionally used to relieve stomach, abdomen, joint, muscle, and rheumatic pain was also reported for its antinociceptive effect on a mouse model. However, the possible pain relief effect of Boesenbergia rotunda ethanolic extract (BREE) via the inhibition to the neural pain pathway remains to be elucidated. This study investigated the inhibitory effect of BREE on compound action potentials (CAPs) and the possible involvement of the opioid receptors. The changes in the CAPs amplitudes of the frog’s sciatic nerves were evaluated following the exposure to three different dosages of BREE (1, 3 and 10 mg/ml and morphine (3 mg/ml). In another set of experiment, the nerves were pretreated with a non-selective opioid receptor antagonist, naloxone (0.1 mg/ml), before exposing the nerve to BREE (1 mg/ml) to investigate the involvement of opioid receptors in the CAPs inhibitory mechanism. The outcome showed a reduction in the CAPs amplitudes when treated with BREE (1, 3 and 10 mg/ml) whereby the effect was reversible. The CAPs inhibition by BREE was absent when the opioid receptors were blocked. Taken together, these findings suggest that BREE-induced CAPs amplitude reduction involves the activation of opioid receptors. Biome Journals 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73296/1/ROTUNDA.pdf Perimal, Enoch Kumar and Chia, Jasmine Siew Min and Gopalsamy, Banulata and Hussain, Mohd Khairi and Omar Farouk, Ahmad Akira and Sulaiman, Mohd Roslan and Amir Ramadan, Ammar Izzati and Wong, Siong Jun and Ghazali, Farihah Hanani (2018) Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors. Life Sciences, Medicine and Biomedicine, 2 (2). art. no. 15. pp. 1-6. ISSN 2600-7207 https://biomescientia.com/index.php/lsmb/article/view/15/11 10.28916/lsmb.2.2.2018.15 |
| spellingShingle | Perimal, Enoch Kumar Chia, Jasmine Siew Min Gopalsamy, Banulata Hussain, Mohd Khairi Omar Farouk, Ahmad Akira Sulaiman, Mohd Roslan Amir Ramadan, Ammar Izzati Wong, Siong Jun Ghazali, Farihah Hanani Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title | Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title_full | Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title_fullStr | Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title_full_unstemmed | Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title_short | Boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| title_sort | boesenbergia rotunda ethanolic extract inhibits compound action potentials via opioid receptors |
| url | http://psasir.upm.edu.my/id/eprint/73296/1/ROTUNDA.pdf |
| work_keys_str_mv | AT perimalenochkumar boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT chiajasminesiewmin boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT gopalsamybanulata boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT hussainmohdkhairi boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT omarfaroukahmadakira boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT sulaimanmohdroslan boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT amirramadanammarizzati boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT wongsiongjun boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors AT ghazalifarihahhanani boesenbergiarotundaethanolicextractinhibitscompoundactionpotentialsviaopioidreceptors |