Genetic characterization of the two colour-type of Kelah
Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three individuals of the kelah were sampled from Sia River of Pahang and Kampung Es...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Malaysian Society of Applied Biology
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7342/1/04_SirajSS.pdf |
| _version_ | 1796967307805720576 |
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| author | Siraj, Siti Shapor Esa, Yuzine Keong, Remmy Bun Poh Daud, Siti Khalijah |
| author_facet | Siraj, Siti Shapor Esa, Yuzine Keong, Remmy Bun Poh Daud, Siti Khalijah |
| author_sort | Siraj, Siti Shapor |
| collection | UPM |
| description | Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three
populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three
individuals of the kelah were sampled from Sia River of Pahang and Kampung Esok River of Negeri Sembilan (silverbronze)and Nenggiri River of Kelantan (reddish). Twelve RAPD primers generated a total of 226 scorable loci with 100% polymorphism across the sixty-three individuals. The RAPD banding patterns and sizes ranged from 4 to 17
and from 100bp to 1500bp, respectively. The intra-population UPGMA dendrogram produced two major clusters,
with the Nenggiri River (Kelantan) samples formed a subcluster in both major clusters dominated by the Pahang
samples (Cluster 1) and N. Sembilan (Cluster 2) samples, respectively. The inter-population UPGMA showed that
the Kelantan samples were genetically closer to the N. Sembilan samples than to the Pahang samples. Thus, the
results of this study did not support the hypothesis that the two colour-types of T. tambroides were genetically distinct from each other. |
| first_indexed | 2024-03-06T07:11:57Z |
| format | Article |
| id | upm.eprints-7342 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:57Z |
| publishDate | 2007 |
| publisher | Malaysian Society of Applied Biology |
| record_format | dspace |
| spelling | upm.eprints-73422013-05-27T07:34:47Z http://psasir.upm.edu.my/id/eprint/7342/ Genetic characterization of the two colour-type of Kelah Siraj, Siti Shapor Esa, Yuzine Keong, Remmy Bun Poh Daud, Siti Khalijah Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three individuals of the kelah were sampled from Sia River of Pahang and Kampung Esok River of Negeri Sembilan (silverbronze)and Nenggiri River of Kelantan (reddish). Twelve RAPD primers generated a total of 226 scorable loci with 100% polymorphism across the sixty-three individuals. The RAPD banding patterns and sizes ranged from 4 to 17 and from 100bp to 1500bp, respectively. The intra-population UPGMA dendrogram produced two major clusters, with the Nenggiri River (Kelantan) samples formed a subcluster in both major clusters dominated by the Pahang samples (Cluster 1) and N. Sembilan (Cluster 2) samples, respectively. The inter-population UPGMA showed that the Kelantan samples were genetically closer to the N. Sembilan samples than to the Pahang samples. Thus, the results of this study did not support the hypothesis that the two colour-types of T. tambroides were genetically distinct from each other. Malaysian Society of Applied Biology 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7342/1/04_SirajSS.pdf Siraj, Siti Shapor and Esa, Yuzine and Keong, Remmy Bun Poh and Daud, Siti Khalijah (2007) Genetic characterization of the two colour-type of Kelah. Malaysian Applied Biololgy, 36 (1). pp. 23-29. ISSN 0126-8643 English |
| spellingShingle | Siraj, Siti Shapor Esa, Yuzine Keong, Remmy Bun Poh Daud, Siti Khalijah Genetic characterization of the two colour-type of Kelah |
| title | Genetic characterization of the two colour-type of Kelah
|
| title_full | Genetic characterization of the two colour-type of Kelah
|
| title_fullStr | Genetic characterization of the two colour-type of Kelah
|
| title_full_unstemmed | Genetic characterization of the two colour-type of Kelah
|
| title_short | Genetic characterization of the two colour-type of Kelah
|
| title_sort | genetic characterization of the two colour type of kelah |
| url | http://psasir.upm.edu.my/id/eprint/7342/1/04_SirajSS.pdf |
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