Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
In this study, endoglucanase was produced from oil palm empty fruit bunch (OPEFB) by a locally isolated aerobic bacterium, Bacillus pumilus EB3. The effects of the fermentation parameters such as initial pH, temperature, and nitrogen source on the endoglucanase production were studied using carboxym...
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| Format: | Artikel |
| Sprache: | English English |
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Elsevier
2008
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| Online Zugang: | http://psasir.upm.edu.my/id/eprint/7353/1/Production%20of%20bacterial%20endoglucanase%20from%20pretreated%20oil%20palm%20empty%20fruit%20bunch%20by%20bacillus%20pumilus%20EB3.pdf |
| _version_ | 1825944191122800640 |
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| author | Ariffin, Hidayah Hassan, Mohd Ali Md Shah, Umi Kalsom Abdullah, Norhafizah Mohamad Ghazali, Farinazleen Shirai, Yoshihito |
| author_facet | Ariffin, Hidayah Hassan, Mohd Ali Md Shah, Umi Kalsom Abdullah, Norhafizah Mohamad Ghazali, Farinazleen Shirai, Yoshihito |
| author_sort | Ariffin, Hidayah |
| collection | UPM |
| description | In this study, endoglucanase was produced from oil palm empty fruit bunch (OPEFB) by a locally isolated aerobic bacterium, Bacillus pumilus EB3. The effects of the fermentation parameters such as initial pH, temperature, and nitrogen source on the endoglucanase production were studied using carboxymethyl cellulose (CMC) as the carbon source. Endoglucanase from B. pumilus EB3 was maximally secreted at 37°C, initial pH 7.0 with 10 g/l of CMC as carbon source, and 2 g/l of yeast extract as organic nitrogen source. The activity recorded during the fermentation was 0.076 U/ml. The productivity of the enzyme increased twofold when 2 g/l of yeast extract was used as the organic nitrogen supplement as compared to the non-supplemented medium. An interesting finding from this study is that pretreated OPEFB medium showed comparable results to CMC medium in terms of enzyme production with an activity of 0.063 U/ml. As OPEFB is an abundant solid waste at palm oil mills, it has the potential of acting as a substrate in cellulase production.
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| first_indexed | 2024-03-06T07:11:59Z |
| format | Article |
| id | upm.eprints-7353 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:59Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-73532015-12-01T07:57:01Z http://psasir.upm.edu.my/id/eprint/7353/ Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3 Ariffin, Hidayah Hassan, Mohd Ali Md Shah, Umi Kalsom Abdullah, Norhafizah Mohamad Ghazali, Farinazleen Shirai, Yoshihito In this study, endoglucanase was produced from oil palm empty fruit bunch (OPEFB) by a locally isolated aerobic bacterium, Bacillus pumilus EB3. The effects of the fermentation parameters such as initial pH, temperature, and nitrogen source on the endoglucanase production were studied using carboxymethyl cellulose (CMC) as the carbon source. Endoglucanase from B. pumilus EB3 was maximally secreted at 37°C, initial pH 7.0 with 10 g/l of CMC as carbon source, and 2 g/l of yeast extract as organic nitrogen source. The activity recorded during the fermentation was 0.076 U/ml. The productivity of the enzyme increased twofold when 2 g/l of yeast extract was used as the organic nitrogen supplement as compared to the non-supplemented medium. An interesting finding from this study is that pretreated OPEFB medium showed comparable results to CMC medium in terms of enzyme production with an activity of 0.063 U/ml. As OPEFB is an abundant solid waste at palm oil mills, it has the potential of acting as a substrate in cellulase production. Elsevier 2008-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7353/1/Production%20of%20bacterial%20endoglucanase%20from%20pretreated%20oil%20palm%20empty%20fruit%20bunch%20by%20bacillus%20pumilus%20EB3.pdf Ariffin, Hidayah and Hassan, Mohd Ali and Md Shah, Umi Kalsom and Abdullah, Norhafizah and Mohamad Ghazali, Farinazleen and Shirai, Yoshihito (2008) Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3. Journal of Bioscience and Bioengineering, 106 (3). pp. 231-236. ISSN 1389-1723 http://dx.doi.org/10.1263/jbb.106.231 10.1263/jbb.106.231 English |
| spellingShingle | Ariffin, Hidayah Hassan, Mohd Ali Md Shah, Umi Kalsom Abdullah, Norhafizah Mohamad Ghazali, Farinazleen Shirai, Yoshihito Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3 |
| title | Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
|
| title_full | Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
|
| title_fullStr | Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
|
| title_full_unstemmed | Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
|
| title_short | Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3
|
| title_sort | production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus eb3 |
| url | http://psasir.upm.edu.my/id/eprint/7353/1/Production%20of%20bacterial%20endoglucanase%20from%20pretreated%20oil%20palm%20empty%20fruit%20bunch%20by%20bacillus%20pumilus%20EB3.pdf |
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