Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation
This study aimed to evaluate the effect of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome as dietary interventions for type 2 diabetes mellitus (T2DM) and its underlying molecular mechanisms in vivo. Experimental rats were induced by high fat diet feeding coupled with combine...
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73551/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20isolated%20from%20Molineria%20latifolia%20ameliorates%20insulin%20resistance%20in%20experimental%20diabetic%20rats%20via%20IRS1AKT%20activation.pdf |
| _version_ | 1796979590301745152 |
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| author | Ooi, Der Jiun Adamu, Hadiza Altine Imam, Mustapha Umar Ithnin, Hairuszah Ismail, Maznah |
| author_facet | Ooi, Der Jiun Adamu, Hadiza Altine Imam, Mustapha Umar Ithnin, Hairuszah Ismail, Maznah |
| author_sort | Ooi, Der Jiun |
| collection | UPM |
| description | This study aimed to evaluate the effect of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome as dietary interventions for type 2 diabetes mellitus (T2DM) and its underlying molecular mechanisms in vivo. Experimental rats were induced by high fat diet feeding coupled with combined exposure to streptozotocin and nicotinamide. Treatment with EAF improved glucose tolerance and lipid profiles, but the insulin secretion was unaltered. Gene expression analyses on insulin/adipocytokine signalling-related genes demonstrated tissue-specific transcriptional responses. In skeletal muscle and liver tissues, Socs1, Tnf and Mapk8 showed consistent transcript regulation. Furthermore, hepatic translational analyses revealed sensitization on proximal insulin signalling, with reduced expression of IRS1 serine phosphorylation, increased IRS1 tyrosine phosphorylation and increased phospho-AKT (Ser473). The present findings suggested that EAF exerted its effect by modulating insulin signalling, potentially via IRS1/AKT activation. The pharmacological attributes of EAF may implicate its potential therapeutic applications for diabetes management. |
| first_indexed | 2024-03-06T10:11:08Z |
| format | Article |
| id | upm.eprints-73551 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:11:08Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-735512020-06-03T03:54:39Z http://psasir.upm.edu.my/id/eprint/73551/ Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation Ooi, Der Jiun Adamu, Hadiza Altine Imam, Mustapha Umar Ithnin, Hairuszah Ismail, Maznah This study aimed to evaluate the effect of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome as dietary interventions for type 2 diabetes mellitus (T2DM) and its underlying molecular mechanisms in vivo. Experimental rats were induced by high fat diet feeding coupled with combined exposure to streptozotocin and nicotinamide. Treatment with EAF improved glucose tolerance and lipid profiles, but the insulin secretion was unaltered. Gene expression analyses on insulin/adipocytokine signalling-related genes demonstrated tissue-specific transcriptional responses. In skeletal muscle and liver tissues, Socs1, Tnf and Mapk8 showed consistent transcript regulation. Furthermore, hepatic translational analyses revealed sensitization on proximal insulin signalling, with reduced expression of IRS1 serine phosphorylation, increased IRS1 tyrosine phosphorylation and increased phospho-AKT (Ser473). The present findings suggested that EAF exerted its effect by modulating insulin signalling, potentially via IRS1/AKT activation. The pharmacological attributes of EAF may implicate its potential therapeutic applications for diabetes management. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73551/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20isolated%20from%20Molineria%20latifolia%20ameliorates%20insulin%20resistance%20in%20experimental%20diabetic%20rats%20via%20IRS1AKT%20activation.pdf Ooi, Der Jiun and Adamu, Hadiza Altine and Imam, Mustapha Umar and Ithnin, Hairuszah and Ismail, Maznah (2018) Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation. Biomedicine & Pharmacotherapy, 98. 125 - 133. ISSN 0753-3322; ESSN: 1950-6007 https://www.sciencedirect.com/science/article/pii/S0753332217338921 10.1016/j.biopha.2017.12.002 |
| spellingShingle | Ooi, Der Jiun Adamu, Hadiza Altine Imam, Mustapha Umar Ithnin, Hairuszah Ismail, Maznah Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title | Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title_full | Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title_fullStr | Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title_full_unstemmed | Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title_short | Polyphenol-rich ethyl acetate fraction isolated from Molineria latifolia ameliorates insulin resistance in experimental diabetic rats via IRS1/AKT activation |
| title_sort | polyphenol rich ethyl acetate fraction isolated from molineria latifolia ameliorates insulin resistance in experimental diabetic rats via irs1 akt activation |
| url | http://psasir.upm.edu.my/id/eprint/73551/1/Polyphenol-rich%20ethyl%20acetate%20fraction%20isolated%20from%20Molineria%20latifolia%20ameliorates%20insulin%20resistance%20in%20experimental%20diabetic%20rats%20via%20IRS1AKT%20activation.pdf |
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