Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9
The plant growth promoting rhizobacteria (PGPR) perform substantial growth enhancement of aerobic rice. Study was conducted at Universiti Putra Malaysia using 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) strategy to identify mechanisms for rice plant growth promotion by PGPR inoculati...
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| Format: | Article |
| Language: | English |
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Pakistan Botanical Society
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73668/1/Proteomic%20study%20on%20growth%20promotion%20of%20PGPR%20inoculated%20aerobic%20rice%20%28Oryza%20sativa%20L.%29%20cultivar%20MR219-9.pdf |
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| author | Naher, Umme Aminun Panhwar, Qurban Ali Othman, Radziah Shamshuddin, Jusop Ismail, Mohd Razi Erxun, Zhou |
| author_facet | Naher, Umme Aminun Panhwar, Qurban Ali Othman, Radziah Shamshuddin, Jusop Ismail, Mohd Razi Erxun, Zhou |
| author_sort | Naher, Umme Aminun |
| collection | UPM |
| description | The plant growth promoting rhizobacteria (PGPR) perform substantial growth enhancement of aerobic rice. Study was conducted at Universiti Putra Malaysia using 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) strategy to identify mechanisms for rice plant growth promotion by PGPR inoculation. In this study, diverse expressed proteins were determined by mass spectrometry (MS). Aerobic rice seedling (MR219-9) was grown in the soil and PGPR strains of Stenotrophomonas maltophilla and Bacillus spp. were inoculated separately and or as combined bacterial consortium. Leaf sheath and other plant parts were collected after 45 d of transplanting for the analysis of proteins. A total of 153 spots were found and from which 12 proteins were identified. All proteins were varied in MS analysis and exposed the differential expression. The identified proteins were tolerant to abiotic stresses (13.2%), disease resistance (10%), oxidation reduction process (10%), photosynthesis (16.62 μmol CO2 m–2 s–1), involved in the protein synthesis (23.28%), metabolism (13.6%) and related to internal plant physiological functions (13.29%). Beside protein identification, phenotypic characters, such as plant height and photosynthetic activity were measured. The highest plant height and length of root, tiller numbers and nutrients uptake were observed in PGPR inoculated treatments. PGPR inoculation increased leaf chlorophyll contents and net photosynthesis rate of inoculated aerobic rice. Hence, using plant proteomic approach it is proved that various designated proteins are responsible for the plant growth promotion of PGPR inoculated aerobic rice cultivar MR219-9. |
| first_indexed | 2024-03-06T10:11:26Z |
| format | Article |
| id | upm.eprints-73668 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:11:26Z |
| publishDate | 2018 |
| publisher | Pakistan Botanical Society |
| record_format | dspace |
| spelling | upm.eprints-736682020-06-24T07:31:31Z http://psasir.upm.edu.my/id/eprint/73668/ Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 Naher, Umme Aminun Panhwar, Qurban Ali Othman, Radziah Shamshuddin, Jusop Ismail, Mohd Razi Erxun, Zhou The plant growth promoting rhizobacteria (PGPR) perform substantial growth enhancement of aerobic rice. Study was conducted at Universiti Putra Malaysia using 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) strategy to identify mechanisms for rice plant growth promotion by PGPR inoculation. In this study, diverse expressed proteins were determined by mass spectrometry (MS). Aerobic rice seedling (MR219-9) was grown in the soil and PGPR strains of Stenotrophomonas maltophilla and Bacillus spp. were inoculated separately and or as combined bacterial consortium. Leaf sheath and other plant parts were collected after 45 d of transplanting for the analysis of proteins. A total of 153 spots were found and from which 12 proteins were identified. All proteins were varied in MS analysis and exposed the differential expression. The identified proteins were tolerant to abiotic stresses (13.2%), disease resistance (10%), oxidation reduction process (10%), photosynthesis (16.62 μmol CO2 m–2 s–1), involved in the protein synthesis (23.28%), metabolism (13.6%) and related to internal plant physiological functions (13.29%). Beside protein identification, phenotypic characters, such as plant height and photosynthetic activity were measured. The highest plant height and length of root, tiller numbers and nutrients uptake were observed in PGPR inoculated treatments. PGPR inoculation increased leaf chlorophyll contents and net photosynthesis rate of inoculated aerobic rice. Hence, using plant proteomic approach it is proved that various designated proteins are responsible for the plant growth promotion of PGPR inoculated aerobic rice cultivar MR219-9. Pakistan Botanical Society 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73668/1/Proteomic%20study%20on%20growth%20promotion%20of%20PGPR%20inoculated%20aerobic%20rice%20%28Oryza%20sativa%20L.%29%20cultivar%20MR219-9.pdf Naher, Umme Aminun and Panhwar, Qurban Ali and Othman, Radziah and Shamshuddin, Jusop and Ismail, Mohd Razi and Erxun, Zhou (2018) Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9. Pakistan Journal of Botany, 50 (5). 1843 - 1852. ISSN 0556-3321 |
| spellingShingle | Naher, Umme Aminun Panhwar, Qurban Ali Othman, Radziah Shamshuddin, Jusop Ismail, Mohd Razi Erxun, Zhou Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title | Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title_full | Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title_fullStr | Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title_full_unstemmed | Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title_short | Proteomic study on growth promotion of PGPR inoculated aerobic rice (Oryza sativa L.) cultivar MR219-9 |
| title_sort | proteomic study on growth promotion of pgpr inoculated aerobic rice oryza sativa l cultivar mr219 9 |
| url | http://psasir.upm.edu.my/id/eprint/73668/1/Proteomic%20study%20on%20growth%20promotion%20of%20PGPR%20inoculated%20aerobic%20rice%20%28Oryza%20sativa%20L.%29%20cultivar%20MR219-9.pdf |
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