Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113
Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Verlag
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/73670/1/Pseudogene%20product%20YqiG%20is%20important%20for%20pflB%20expression%20and%20biohydrogen%20production%20in%20Escherichia%20coli%20BW25113.pdf |
| _version_ | 1825950230401515520 |
|---|---|
| author | Zakaria, Muhammad Azman Mohd Yusoff, Mohd Zulkhairi Zakaria @ Mamat, Mohd Rafein Hassan, Mohd Ali Wood, Thomas K. Maeda, Toshinari |
| author_facet | Zakaria, Muhammad Azman Mohd Yusoff, Mohd Zulkhairi Zakaria @ Mamat, Mohd Rafein Hassan, Mohd Ali Wood, Thomas K. Maeda, Toshinari |
| author_sort | Zakaria, Muhammad Azman |
| collection | UPM |
| description | Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg−1 protein to 6 µmol mg−1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli. |
| first_indexed | 2024-03-06T10:11:26Z |
| format | Article |
| id | upm.eprints-73670 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:11:26Z |
| publishDate | 2018 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | upm.eprints-736702020-06-24T07:40:13Z http://psasir.upm.edu.my/id/eprint/73670/ Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 Zakaria, Muhammad Azman Mohd Yusoff, Mohd Zulkhairi Zakaria @ Mamat, Mohd Rafein Hassan, Mohd Ali Wood, Thomas K. Maeda, Toshinari Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg−1 protein to 6 µmol mg−1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli. Springer Verlag 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73670/1/Pseudogene%20product%20YqiG%20is%20important%20for%20pflB%20expression%20and%20biohydrogen%20production%20in%20Escherichia%20coli%20BW25113.pdf Zakaria, Muhammad Azman and Mohd Yusoff, Mohd Zulkhairi and Zakaria @ Mamat, Mohd Rafein and Hassan, Mohd Ali and Wood, Thomas K. and Maeda, Toshinari (2018) Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113. 3 Biotech, 8 (10). pp. 1-8. ISSN 2190-572X; ESSN: 2190-5738 https://link.springer.com/article/10.1007/s13205-018-1461-2 10.1007/s13205-018-1461-2 |
| spellingShingle | Zakaria, Muhammad Azman Mohd Yusoff, Mohd Zulkhairi Zakaria @ Mamat, Mohd Rafein Hassan, Mohd Ali Wood, Thomas K. Maeda, Toshinari Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title | Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title_full | Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title_fullStr | Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title_full_unstemmed | Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title_short | Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113 |
| title_sort | pseudogene product yqig is important for pflb expression and biohydrogen production in escherichia coli bw25113 |
| url | http://psasir.upm.edu.my/id/eprint/73670/1/Pseudogene%20product%20YqiG%20is%20important%20for%20pflB%20expression%20and%20biohydrogen%20production%20in%20Escherichia%20coli%20BW25113.pdf |
| work_keys_str_mv | AT zakariamuhammadazman pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 AT mohdyusoffmohdzulkhairi pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 AT zakariamamatmohdrafein pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 AT hassanmohdali pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 AT woodthomask pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 AT maedatoshinari pseudogeneproductyqigisimportantforpflbexpressionandbiohydrogenproductioninescherichiacolibw25113 |