Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen
Background: Hepatitis B surface antigen is usually secreted by infected hepatocytes in the form of subviral particles rather than infectious virions, while the hepatitis B e antigen originates from the core gene and is modified and secreted by hepatocytes into the circulation and functions as a mark...
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| Format: | Article |
| Language: | English |
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Clinical Laboratory Publications
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/73697/1/Quantitative%20hepatitis%20B%20e%20antigen%20a%20better%20predictor%20of%20hepatitis%20B%20virus%20DNA%20than%20quantitative%20hepatitis%20B%20surface%20antigen.pdf |
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| author | Hudu, Shuaibu A. Mohd Taib, Niazlin Amin Nordin, Syafinaz Saeed, Mohammed I. Tan, Soek S. Omar, Haniza Shahar, Hamiza Sekawi, Zamberi |
| author_facet | Hudu, Shuaibu A. Mohd Taib, Niazlin Amin Nordin, Syafinaz Saeed, Mohammed I. Tan, Soek S. Omar, Haniza Shahar, Hamiza Sekawi, Zamberi |
| author_sort | Hudu, Shuaibu A. |
| collection | UPM |
| description | Background: Hepatitis B surface antigen is usually secreted by infected hepatocytes in the form of subviral particles rather than infectious virions, while the hepatitis B e antigen originates from the core gene and is modified and secreted by hepatocytes into the circulation and functions as a marker of active viral replication. This study aimed to study the relationship between HBV DNA and quantitative hepatitis B surface and e antigen in Malaysian patients. Methods: A total of 82 chronic hepatitis B patients were recruited for this study from the Hepatology Department of Selayang Hospital. Quantitative hepatitis surface and e antigen was performed retrospectively on frozen plasma using enzyme linked immunosorbent assay according to the manufacturer's instructions. Hepatitis B viral DNA was extracted from all plasma samples and quantified using real-time PCR. Results: Quantitative hepatitis B surface and e antigens were found be high in 54.9% and 52.4% of the patients, respectively, while hepatitis B virus DNA level was high in 70.7%. The median of the viral load of HBV was 8,934.89 IU/mL and both hepatitis B surface and e antigens were also found to be high on average for qHBsAg (M = 5.19 IU/mL, SD ± 4. 33) and qHBeAg (M = 4.74IU/mL, SD ± 4.20), with qHBeAg being more strongly correlated to HBV DNA than qHBsAg (r = 0.893; p < 0.01). Conclusions: This study revealed HBeAg to be the most appropriate marker that correlates well with HBV DNA, thus not completely novel but confirmative, and related to the Malaysian situation. |
| first_indexed | 2024-03-06T10:11:31Z |
| format | Article |
| id | upm.eprints-73697 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:11:31Z |
| publishDate | 2018 |
| publisher | Clinical Laboratory Publications |
| record_format | dspace |
| spelling | upm.eprints-736972020-05-12T05:53:47Z http://psasir.upm.edu.my/id/eprint/73697/ Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen Hudu, Shuaibu A. Mohd Taib, Niazlin Amin Nordin, Syafinaz Saeed, Mohammed I. Tan, Soek S. Omar, Haniza Shahar, Hamiza Sekawi, Zamberi Background: Hepatitis B surface antigen is usually secreted by infected hepatocytes in the form of subviral particles rather than infectious virions, while the hepatitis B e antigen originates from the core gene and is modified and secreted by hepatocytes into the circulation and functions as a marker of active viral replication. This study aimed to study the relationship between HBV DNA and quantitative hepatitis B surface and e antigen in Malaysian patients. Methods: A total of 82 chronic hepatitis B patients were recruited for this study from the Hepatology Department of Selayang Hospital. Quantitative hepatitis surface and e antigen was performed retrospectively on frozen plasma using enzyme linked immunosorbent assay according to the manufacturer's instructions. Hepatitis B viral DNA was extracted from all plasma samples and quantified using real-time PCR. Results: Quantitative hepatitis B surface and e antigens were found be high in 54.9% and 52.4% of the patients, respectively, while hepatitis B virus DNA level was high in 70.7%. The median of the viral load of HBV was 8,934.89 IU/mL and both hepatitis B surface and e antigens were also found to be high on average for qHBsAg (M = 5.19 IU/mL, SD ± 4. 33) and qHBeAg (M = 4.74IU/mL, SD ± 4.20), with qHBeAg being more strongly correlated to HBV DNA than qHBsAg (r = 0.893; p < 0.01). Conclusions: This study revealed HBeAg to be the most appropriate marker that correlates well with HBV DNA, thus not completely novel but confirmative, and related to the Malaysian situation. Clinical Laboratory Publications 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73697/1/Quantitative%20hepatitis%20B%20e%20antigen%20a%20better%20predictor%20of%20hepatitis%20B%20virus%20DNA%20than%20quantitative%20hepatitis%20B%20surface%20antigen.pdf Hudu, Shuaibu A. and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Saeed, Mohammed I. and Tan, Soek S. and Omar, Haniza and Shahar, Hamiza and Sekawi, Zamberi (2018) Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen. Clinical Laboratory, 64 (4). 443 - 449. ISSN 1433-6510 10.7754/Clin.Lab.2017.170916 |
| spellingShingle | Hudu, Shuaibu A. Mohd Taib, Niazlin Amin Nordin, Syafinaz Saeed, Mohammed I. Tan, Soek S. Omar, Haniza Shahar, Hamiza Sekawi, Zamberi Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title | Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title_full | Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title_fullStr | Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title_full_unstemmed | Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title_short | Quantitative hepatitis B e antigen: a better predictor of hepatitis B virus DNA than quantitative hepatitis B surface antigen |
| title_sort | quantitative hepatitis b e antigen a better predictor of hepatitis b virus dna than quantitative hepatitis b surface antigen |
| url | http://psasir.upm.edu.my/id/eprint/73697/1/Quantitative%20hepatitis%20B%20e%20antigen%20a%20better%20predictor%20of%20hepatitis%20B%20virus%20DNA%20than%20quantitative%20hepatitis%20B%20surface%20antigen.pdf |
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