Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography
Hepatitis B core antigen with the mutation of Y132A (HBcAg-Y132A) was successfully expressed in Escherichia coli. The mutant HBcAg-Y132A forms dimers and is unable to self-assemble into virus-like particles (VLPs). Hence, it is a potential antigen used in the antibody-responsive biosensor for the de...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2018
|
| _version_ | 1825950269067755520 |
|---|---|
| author | lim, Swee Lu Ng, Hong Wei Akwiditya, Made Angga Ooi, Chien Wei Chan, Eng Seng Ho, Kok Lian Tan, Wen Siang Chua, Gek Kee Tey, Beng Ti |
| author_facet | lim, Swee Lu Ng, Hong Wei Akwiditya, Made Angga Ooi, Chien Wei Chan, Eng Seng Ho, Kok Lian Tan, Wen Siang Chua, Gek Kee Tey, Beng Ti |
| author_sort | lim, Swee Lu |
| collection | UPM |
| description | Hepatitis B core antigen with the mutation of Y132A (HBcAg-Y132A) was successfully expressed in Escherichia coli. The mutant HBcAg-Y132A forms dimers and is unable to self-assemble into virus-like particles (VLPs). Hence, it is a potential antigen used in the antibody-responsive biosensor for the detection of anti-HBcAg in patients infected with hepatitis B virus. The aim of this study was to establish a direct purification strategy to recover HBcAg-Y132A dimer from the E. coli feedstock using SepFast™ Supor DEAE pre-packed column. The performance of this anion exchange chromatography was optimized in terms of the buffer composition (for adsorption and elution steps) and the mode of elution (i.e., step or gradient). The highest adsorption of HBcAg-Y132A dimer in the DEAE column was achieved with the buffer composed of 50 mM Tris-HCl (pH 8.4). The step elution using 50 mM Tris-HCl elution buffer (pH 8.4) supplemented with 1 M NaCl resulted in 1.2-fold increase in the purity of HBcAg, as compared to the gradient elution mode. In addition, it was found that the optimized 3-step elution is not directly applicable to elute the self-assembled HBcAg VLPs, as only 24.7% of the particles were recovered due to the limitation of size effect. |
| first_indexed | 2024-03-06T10:12:04Z |
| format | Article |
| id | upm.eprints-73900 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:12:04Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-739002022-11-23T04:10:28Z http://psasir.upm.edu.my/id/eprint/73900/ Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography lim, Swee Lu Ng, Hong Wei Akwiditya, Made Angga Ooi, Chien Wei Chan, Eng Seng Ho, Kok Lian Tan, Wen Siang Chua, Gek Kee Tey, Beng Ti Hepatitis B core antigen with the mutation of Y132A (HBcAg-Y132A) was successfully expressed in Escherichia coli. The mutant HBcAg-Y132A forms dimers and is unable to self-assemble into virus-like particles (VLPs). Hence, it is a potential antigen used in the antibody-responsive biosensor for the detection of anti-HBcAg in patients infected with hepatitis B virus. The aim of this study was to establish a direct purification strategy to recover HBcAg-Y132A dimer from the E. coli feedstock using SepFast™ Supor DEAE pre-packed column. The performance of this anion exchange chromatography was optimized in terms of the buffer composition (for adsorption and elution steps) and the mode of elution (i.e., step or gradient). The highest adsorption of HBcAg-Y132A dimer in the DEAE column was achieved with the buffer composed of 50 mM Tris-HCl (pH 8.4). The step elution using 50 mM Tris-HCl elution buffer (pH 8.4) supplemented with 1 M NaCl resulted in 1.2-fold increase in the purity of HBcAg, as compared to the gradient elution mode. In addition, it was found that the optimized 3-step elution is not directly applicable to elute the self-assembled HBcAg VLPs, as only 24.7% of the particles were recovered due to the limitation of size effect. Elsevier 2018-06 Article PeerReviewed lim, Swee Lu and Ng, Hong Wei and Akwiditya, Made Angga and Ooi, Chien Wei and Chan, Eng Seng and Ho, Kok Lian and Tan, Wen Siang and Chua, Gek Kee and Tey, Beng Ti (2018) Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography. Process Biochemistry, 69. 208 - 215. ISSN 1359-5113 https://www.sciencedirect.com/science/article/pii/S1359511317311777 10.1016/j.procbio.2018.03.003 |
| spellingShingle | lim, Swee Lu Ng, Hong Wei Akwiditya, Made Angga Ooi, Chien Wei Chan, Eng Seng Ho, Kok Lian Tan, Wen Siang Chua, Gek Kee Tey, Beng Ti Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title | Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title_full | Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title_fullStr | Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title_full_unstemmed | Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title_short | Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography |
| title_sort | single step purification of recombinant hepatitis b core antigen y132a dimer from clarified escherichia coli feedstock using a packed bed anion exchange chromatography |
| work_keys_str_mv | AT limsweelu singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT nghongwei singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT akwidityamadeangga singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT ooichienwei singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT chanengseng singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT hokoklian singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT tanwensiang singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT chuagekkee singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography AT teybengti singlesteppurificationofrecombinanthepatitisbcoreantigeny132adimerfromclarifiedescherichiacolifeedstockusingapackedbedanionexchangechromatography |