Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography

Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography

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Hepatitis B core antigen with the mutation of Y132A (HBcAg-Y132A) was successfully expressed in Escherichia coli. The mutant HBcAg-Y132A forms dimers and is unable to self-assemble into virus-like particles (VLPs). Hence, it is a potential antigen used in the antibody-responsive biosensor for the de...

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Bibliographic Details
Main Authors:	lim, Swee Lu, Ng, Hong Wei, Akwiditya, Made Angga, Ooi, Chien Wei, Chan, Eng Seng, Ho, Kok Lian, Tan, Wen Siang, Chua, Gek Kee, Tey, Beng Ti
Format:	Article
Published:	Elsevier 2018

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