The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells
The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Biome Scientia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf |
| _version_ | 1825950302254137344 |
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| author | Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar |
| author_facet | Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar |
| author_sort | Mohammed Izham, Aishah |
| collection | UPM |
| description | The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research is due to their resemblance to the human primary neuronal cells with several limitations, considering the cells are derived from malignant tumor cells. Since SH-SY5Y cells are widely cultured for research, several different culture media have been used to optimize the growth of the cells, including Eagle's Minimum Essential Medium (EMEM), Dulbecco’s modified Eagle’s medium (DMEM) and other recently developed culture media. The ability of SH-SY5Y cells to reach confluency in culture flasks ranges from 5 days to 15 days, depending on the culture media. Hence, the optimization of the culture media is crucial to achieve the fastest growth rate for the cells. The objective of the study is to evaluate the culture media for the proliferation of SH-SY5Y cells. We compared the growth rate of SH-SY5Y cells cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% heat-inactivated fetal bovine serum (hiFBS), Dulbecco’s modified Eagle’s medium: Nutrientmixture F-12 (DMEM:F12) + supplemented with 15% hiFBS and DMEM:F12 supplemented with 10% hiFBS. In DMEM:F12 supplemented with 15% hiFBS, cells grew up to 6.67E+05 cells. In DMEM:F12 supplemented with 10%hiFBS, cells grew up to 5.28E+05 cells. In DMEM supplemented with 15% hiFBS, the cells grew up to 4.76E+05 cells. There was a significant difference between culture media DMEM:F12 supplemented with 15% hiFBS as compared to DMEM:F12 supplemented with 10%hiFBS and DMEM supplemented with 15% hiFBS (p0.05). |
| first_indexed | 2024-03-06T10:12:34Z |
| format | Article |
| id | upm.eprints-74142 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:12:34Z |
| publishDate | 2018 |
| publisher | Biome Scientia |
| record_format | dspace |
| spelling | upm.eprints-741422021-09-22T21:30:21Z http://psasir.upm.edu.my/id/eprint/74142/ The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar The human neuroblastoma cell line, SH-SY5Y cells, is commonly used as an in vitro model for neuroscience and neurobiology research. SH-SY5Y cells were established during the 1970’s from the parental metastatic bone tumor, SK-N-SH cell line. The utilization of SH-SY5Y cells in neurobiology research is due to their resemblance to the human primary neuronal cells with several limitations, considering the cells are derived from malignant tumor cells. Since SH-SY5Y cells are widely cultured for research, several different culture media have been used to optimize the growth of the cells, including Eagle's Minimum Essential Medium (EMEM), Dulbecco’s modified Eagle’s medium (DMEM) and other recently developed culture media. The ability of SH-SY5Y cells to reach confluency in culture flasks ranges from 5 days to 15 days, depending on the culture media. Hence, the optimization of the culture media is crucial to achieve the fastest growth rate for the cells. The objective of the study is to evaluate the culture media for the proliferation of SH-SY5Y cells. We compared the growth rate of SH-SY5Y cells cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% heat-inactivated fetal bovine serum (hiFBS), Dulbecco’s modified Eagle’s medium: Nutrientmixture F-12 (DMEM:F12) + supplemented with 15% hiFBS and DMEM:F12 supplemented with 10% hiFBS. In DMEM:F12 supplemented with 15% hiFBS, cells grew up to 6.67E+05 cells. In DMEM:F12 supplemented with 10%hiFBS, cells grew up to 5.28E+05 cells. In DMEM supplemented with 15% hiFBS, the cells grew up to 4.76E+05 cells. There was a significant difference between culture media DMEM:F12 supplemented with 15% hiFBS as compared to DMEM:F12 supplemented with 10%hiFBS and DMEM supplemented with 15% hiFBS (p0.05). Biome Scientia 2018-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf Mohammed Izham, Aishah and Chia, Jasmine Siew Min and Vidyadaran, Sharmili and Sulaiman, Mohd Roslan and Bharatham, Hemabarathy and Perimal, Enoch Kumar (2018) The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells. Life Sciences, Medicine and Biomedicine, 2 (3). art. no. 23. pp. 1-4. ISSN 2600-7207 https://biomescientia.com/index.php/lsmb/article/view/23 https://doi.org/10.28916/lsmb.2.3.2018.23 |
| spellingShingle | Mohammed Izham, Aishah Chia, Jasmine Siew Min Vidyadaran, Sharmili Sulaiman, Mohd Roslan Bharatham, Hemabarathy Perimal, Enoch Kumar The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title | The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title_full | The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title_fullStr | The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title_full_unstemmed | The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title_short | The effect of DMEM and DMEM:F12 culture media on the growth of SH-SY5Y cells |
| title_sort | effect of dmem and dmem f12 culture media on the growth of sh sy5y cells |
| url | http://psasir.upm.edu.my/id/eprint/74142/1/The%20effect%20of%20DMEM%20and%20DMEM.pdf |
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