The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. How...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Penerbit UKM
2018
|
| _version_ | 1796979687996522496 |
|---|---|
| author | Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd Yusuf, Yusmin tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani |
| author_facet | Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd Yusuf, Yusmin tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani |
| author_sort | Baharum, Nadiya Akmal |
| collection | UPM |
| description | PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over- expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants. |
| first_indexed | 2024-03-06T10:12:35Z |
| format | Article |
| id | upm.eprints-74149 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:12:35Z |
| publishDate | 2018 |
| publisher | Penerbit UKM |
| record_format | dspace |
| spelling | upm.eprints-741492023-03-15T02:28:49Z http://psasir.upm.edu.my/id/eprint/74149/ The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd Yusuf, Yusmin tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over- expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants. Penerbit UKM 2018-10 Article PeerReviewed Baharum, Nadiya Akmal and Othman, Rofina Yasmin and Mohd Yusuf, Yusmin and tan, Boon Chin and Zaidi, Kamilatulhusna and Khalid, Norzulaani (2018) The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan. Sains Malaysiana, 47 (10). 2291 - 2300. ISSN 0126-6039; ESSN: 2735-0188 https://www.ukm.my/jsm/english_journals/vol47num10_2018/contentsVol47num10_2018.htm 10.17576/jsm-2018-4710-05 |
| spellingShingle | Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd Yusuf, Yusmin tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title | The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title_full | The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title_fullStr | The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title_full_unstemmed | The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title_short | The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan |
| title_sort | effect of pathogenesis related 10 pr 10 gene on the progression of fusarium wilt in musa acuminata cv berangan |
| work_keys_str_mv | AT baharumnadiyaakmal theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT othmanrofinayasmin theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT mohdyusufyusmin theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT tanboonchin theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT zaidikamilatulhusna theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT khalidnorzulaani theeffectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT baharumnadiyaakmal effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT othmanrofinayasmin effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT mohdyusufyusmin effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT tanboonchin effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT zaidikamilatulhusna effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan AT khalidnorzulaani effectofpathogenesisrelated10pr10geneontheprogressionoffusariumwiltinmusaacuminatacvberangan |