The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons
Polycyclic aromatic hydrocarbons are pollutants which are persistent in nature. The aryl hydrocarbon receptor is a ligand-activated cytosolic transcription factor activated by xenobiotics. The objective was to isolate and identify AHR mRNA transcript in immune organs of developing chicks and to inte...
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| Format: | Article |
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Taylor and Francis
2018
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| author | Nisha, A. R. Hazilawati, H. Mohd Azmi, M. L. Noordin, M. M. |
| author_facet | Nisha, A. R. Hazilawati, H. Mohd Azmi, M. L. Noordin, M. M. |
| author_sort | Nisha, A. R. |
| collection | UPM |
| description | Polycyclic aromatic hydrocarbons are pollutants which are persistent in nature. The aryl hydrocarbon receptor is a ligand-activated cytosolic transcription factor activated by xenobiotics. The objective was to isolate and identify AHR mRNA transcript in immune organs of developing chicks and to interpret the correlation between AHR induction and dose of PAHs. Specific pathogen free embryonated eggs on day nine were inoculated with solutions of pyrene, phenanthrene, and fluoranthene dissolved in tricaprylin (vehicle) through the allantoic route at three dose levels: 0.2 mg/kg, 2 mg/kg, and 20 mg/kg. A 650 base pair product was observed by RNA extraction and reverse transcription PCR from thymus, bursa of Fabricius and spleen on 21st day. When AHR concentration was analyzed by ELISA in these organs, pyrene showed maximum potency in inducing AHR in thymus. Fluoranthene made highest concentration of AHR in bursa of Fabricius. None of these chemicals caused an increase in AHR concentration in spleen. |
| first_indexed | 2024-09-25T03:35:11Z |
| format | Article |
| id | upm.eprints-74209 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:35:11Z |
| publishDate | 2018 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | upm.eprints-742092024-09-11T01:40:39Z http://psasir.upm.edu.my/id/eprint/74209/ The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons Nisha, A. R. Hazilawati, H. Mohd Azmi, M. L. Noordin, M. M. Polycyclic aromatic hydrocarbons are pollutants which are persistent in nature. The aryl hydrocarbon receptor is a ligand-activated cytosolic transcription factor activated by xenobiotics. The objective was to isolate and identify AHR mRNA transcript in immune organs of developing chicks and to interpret the correlation between AHR induction and dose of PAHs. Specific pathogen free embryonated eggs on day nine were inoculated with solutions of pyrene, phenanthrene, and fluoranthene dissolved in tricaprylin (vehicle) through the allantoic route at three dose levels: 0.2 mg/kg, 2 mg/kg, and 20 mg/kg. A 650 base pair product was observed by RNA extraction and reverse transcription PCR from thymus, bursa of Fabricius and spleen on 21st day. When AHR concentration was analyzed by ELISA in these organs, pyrene showed maximum potency in inducing AHR in thymus. Fluoranthene made highest concentration of AHR in bursa of Fabricius. None of these chemicals caused an increase in AHR concentration in spleen. Taylor and Francis 2018 Article PeerReviewed Nisha, A. R. and Hazilawati, H. and Mohd Azmi, M. L. and Noordin, M. M. (2018) The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons. Toxicology Mechanisms and Methods, 28 (6). pp. 461-466. ISSN 1537-6516; EISSN: 1537-6524 https://www.tandfonline.com/doi/full/10.1080/15376516.2018.1459992 10.1080/15376516.2018.1459992 |
| spellingShingle | Nisha, A. R. Hazilawati, H. Mohd Azmi, M. L. Noordin, M. M. The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title | The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title_full | The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title_fullStr | The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title_full_unstemmed | The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title_short | The induction of aryl hydrocarbon receptor (AHR) in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
| title_sort | induction of aryl hydrocarbon receptor ahr in immune organs of developing chicks by polycyclic aromatic hydrocarbons |
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