Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract
The present study aims for the first time to provide the in vivo acute toxicological profile of the highest dose of Clinacanthus nutans (Burm. f.) Lindau water leaf extract according to the Organization for economic co-operation and development (OECD) 423 guidelines through conventional toxicity and...
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| Format: | Article |
| Language: | English |
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MDPI
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74501/1/Hematological%2C%20biochemical%2C%20histopathological%20and%201H-NMR%20metabolomics%20application%20in%20acute%20toxicity%20evaluation%20of%20clinacanthus%20nutans%20water%20leaf%20extract.pdf |
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| author | Leng, Wei Khoo Siew, Audrey Foong Kow M., Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah |
| author_facet | Leng, Wei Khoo Siew, Audrey Foong Kow M., Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah |
| author_sort | Leng, Wei Khoo |
| collection | UPM |
| description | The present study aims for the first time to provide the in vivo acute toxicological profile of the highest dose of Clinacanthus nutans (Burm. f.) Lindau water leaf extract according to the Organization for economic co-operation and development (OECD) 423 guidelines through conventional toxicity and advanced proton nuclear magnetic resonance (1H-NMR) serum and urinary metabolomics evaluation methods. A single dose of 5000 mg/kg bw of C. nutans water extract was administered to Sprague Dawley rats, and they were observed for 14 days. Conventional toxicity evaluation methods (physical observation, body and organ weight, food and water consumption, hematology, biochemical testing and histopathological analysis) suggested no abnormal toxicity signs. Serum 1H-NMR metabolome revealed no significant metabolic difference between untreated and treated groups. Urinary 1H-NMR analysis, on the other hand, revealed alteration in carbohydrate metabolism, energy metabolism and amino acid metabolism in extract-treated rats after 2 h of extract administration, but the metabolic expression collected after 24 h and at Day 5, Day 10 and Day 15 indicated that the extract-treated rats did not accumulate any toxicity biomarkers. Importantly, the outcomes further suggest that single oral administration of up to 5000 mg/kg bw of C. nutans water leaf extract is safe for consumption. |
| first_indexed | 2024-03-06T10:13:13Z |
| format | Article |
| id | upm.eprints-74501 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:13:13Z |
| publishDate | 2018 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-745012020-10-16T05:59:05Z http://psasir.upm.edu.my/id/eprint/74501/ Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract Leng, Wei Khoo Siew, Audrey Foong Kow M., Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah The present study aims for the first time to provide the in vivo acute toxicological profile of the highest dose of Clinacanthus nutans (Burm. f.) Lindau water leaf extract according to the Organization for economic co-operation and development (OECD) 423 guidelines through conventional toxicity and advanced proton nuclear magnetic resonance (1H-NMR) serum and urinary metabolomics evaluation methods. A single dose of 5000 mg/kg bw of C. nutans water extract was administered to Sprague Dawley rats, and they were observed for 14 days. Conventional toxicity evaluation methods (physical observation, body and organ weight, food and water consumption, hematology, biochemical testing and histopathological analysis) suggested no abnormal toxicity signs. Serum 1H-NMR metabolome revealed no significant metabolic difference between untreated and treated groups. Urinary 1H-NMR analysis, on the other hand, revealed alteration in carbohydrate metabolism, energy metabolism and amino acid metabolism in extract-treated rats after 2 h of extract administration, but the metabolic expression collected after 24 h and at Day 5, Day 10 and Day 15 indicated that the extract-treated rats did not accumulate any toxicity biomarkers. Importantly, the outcomes further suggest that single oral administration of up to 5000 mg/kg bw of C. nutans water leaf extract is safe for consumption. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74501/1/Hematological%2C%20biochemical%2C%20histopathological%20and%201H-NMR%20metabolomics%20application%20in%20acute%20toxicity%20evaluation%20of%20clinacanthus%20nutans%20water%20leaf%20extract.pdf Leng, Wei Khoo and Siew, Audrey Foong Kow and M., Maulidiani and Lee, Ming Tatt and Tan, Chin Ping and Shaari, Khozirah and Tham, Chau Ling and Abas, Faridah (2018) Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract. Molecules, 23 (9). art. no. 2172. pp. 1-18. ISSN 1420-3049 https://pubmed.ncbi.nlm.nih.gov/30158427/ 10.3390/molecules23092172 |
| spellingShingle | Leng, Wei Khoo Siew, Audrey Foong Kow M., Maulidiani Lee, Ming Tatt Tan, Chin Ping Shaari, Khozirah Tham, Chau Ling Abas, Faridah Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title | Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title_full | Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title_fullStr | Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title_full_unstemmed | Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title_short | Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| title_sort | hematological biochemical histopathological and 1h nmr metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract |
| url | http://psasir.upm.edu.my/id/eprint/74501/1/Hematological%2C%20biochemical%2C%20histopathological%20and%201H-NMR%20metabolomics%20application%20in%20acute%20toxicity%20evaluation%20of%20clinacanthus%20nutans%20water%20leaf%20extract.pdf |
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