Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region
Nuclear ribosomal internal transcribed spacer (ITS) sequences and morphological characteristics were used to identify medicinally important Kaempferia species cultivated in Peninsular Malaysia. Six species were evaluated for parameters such as nucleotide diversity (0.458) and estimated values of tra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74539/1/Identification.pdf |
| _version_ | 1796979735846191104 |
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| author | Labrooy, Catherine Dharshini Abdullah, Thohirah Lee Stanslas, Johnson |
| author_facet | Labrooy, Catherine Dharshini Abdullah, Thohirah Lee Stanslas, Johnson |
| author_sort | Labrooy, Catherine Dharshini |
| collection | UPM |
| description | Nuclear ribosomal internal transcribed spacer (ITS) sequences and morphological characteristics were used to identify medicinally important Kaempferia species cultivated in Peninsular Malaysia. Six species were evaluated for parameters such as nucleotide diversity (0.458) and estimated values of transition/transversion bias (0.89) using sequence data. Maximum parsimony (MP) analysis inferred divergence pattern in the Kaempferia genus. The resulting phylogenetic tree was compared to one formed using morphological traits. Morphological and molecular data both show 3 distinctive groupings within selected Kaempferia species. ITS 4 and 5 sequences are proposed as DNA barcodes for identification of Kaempferia species. In terms of morphological traits, plant habit, rhizome colour and leaf variation can be used for preliminary identification of this genus. |
| first_indexed | 2024-03-06T10:13:17Z |
| format | Article |
| id | upm.eprints-74539 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:13:17Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-745392020-02-26T09:33:05Z http://psasir.upm.edu.my/id/eprint/74539/ Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region Labrooy, Catherine Dharshini Abdullah, Thohirah Lee Stanslas, Johnson Nuclear ribosomal internal transcribed spacer (ITS) sequences and morphological characteristics were used to identify medicinally important Kaempferia species cultivated in Peninsular Malaysia. Six species were evaluated for parameters such as nucleotide diversity (0.458) and estimated values of transition/transversion bias (0.89) using sequence data. Maximum parsimony (MP) analysis inferred divergence pattern in the Kaempferia genus. The resulting phylogenetic tree was compared to one formed using morphological traits. Morphological and molecular data both show 3 distinctive groupings within selected Kaempferia species. ITS 4 and 5 sequences are proposed as DNA barcodes for identification of Kaempferia species. In terms of morphological traits, plant habit, rhizome colour and leaf variation can be used for preliminary identification of this genus. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74539/1/Identification.pdf Labrooy, Catherine Dharshini and Abdullah, Thohirah Lee and Stanslas, Johnson (2018) Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region. Current Plant Biology, 14. 50 - 55. ISSN 2214-6628 10.1016/j.cpb.2018.09.004 |
| spellingShingle | Labrooy, Catherine Dharshini Abdullah, Thohirah Lee Stanslas, Johnson Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title | Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title_full | Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title_fullStr | Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title_full_unstemmed | Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title_short | Identification of ethnomedicinally important Kaempferia L. (Zingiberaceae) species based on morphological traits and suitable DNA region |
| title_sort | identification of ethnomedicinally important kaempferia l zingiberaceae species based on morphological traits and suitable dna region |
| url | http://psasir.upm.edu.my/id/eprint/74539/1/Identification.pdf |
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