Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities
Ficus deltoidea is a traditional medicinal plant that has been proven to show antidiabetic effects. This study focus is to assess the insulin secretion activity of Ficus deltoidea standardized methanolic extracts from seven independent varieties and mechanisms that underlie the insulin secretion act...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74701/1/Insulinotropic%20activity.pdf |
| _version_ | 1825950369554890752 |
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| author | Yahaya, Nurshieren Mohd Dom, Nur Sumirah Adam, Zainah Hamid, Muhajir |
| author_facet | Yahaya, Nurshieren Mohd Dom, Nur Sumirah Adam, Zainah Hamid, Muhajir |
| author_sort | Yahaya, Nurshieren |
| collection | UPM |
| description | Ficus deltoidea is a traditional medicinal plant that has been proven to show antidiabetic effects. This study focus is to assess the insulin secretion activity of Ficus deltoidea standardized methanolic extracts from seven independent varieties and mechanisms that underlie the insulin secretion action of the extracts. The cytotoxicity of Ficus deltoidea extracts was tested using viability assay. The insulin secretion assay was carried out by treating clonal BRIN BD11 cell line with standardized methanolic Ficus deltoidea extracts or glybenclamide. The clonal BRIN BD11 cell was also treated with insulin agonist and antagonist to elucidate the insulin secretion mechanism. Only the viability percentage for Ficus deltoidea var. kunstleri and intermedia was identified to be toxic at 500 and 1000 μg/ml (P<0.001). The insulin secretion for Ficus deltoidea var. deltoidea, angustifolia, and motleyana was dose-dependent; further evaluation suggested that Ficus deltoidea var. trengganuensis was involved in K ᴬᵀᴾ -independent pathway. This study suggests that standardized methanolic extracts of Ficus deltoidea varieties have an insulinotropic effect on clonal BRIN BD11 cell line and can be utilized as a modern candidate of antidiabetic agents targeting the escalation for insulin secretion from pancreatic beta cells. |
| first_indexed | 2024-03-06T10:13:33Z |
| format | Article |
| id | upm.eprints-74701 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:13:33Z |
| publishDate | 2018 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | upm.eprints-747012020-02-27T09:21:38Z http://psasir.upm.edu.my/id/eprint/74701/ Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities Yahaya, Nurshieren Mohd Dom, Nur Sumirah Adam, Zainah Hamid, Muhajir Ficus deltoidea is a traditional medicinal plant that has been proven to show antidiabetic effects. This study focus is to assess the insulin secretion activity of Ficus deltoidea standardized methanolic extracts from seven independent varieties and mechanisms that underlie the insulin secretion action of the extracts. The cytotoxicity of Ficus deltoidea extracts was tested using viability assay. The insulin secretion assay was carried out by treating clonal BRIN BD11 cell line with standardized methanolic Ficus deltoidea extracts or glybenclamide. The clonal BRIN BD11 cell was also treated with insulin agonist and antagonist to elucidate the insulin secretion mechanism. Only the viability percentage for Ficus deltoidea var. kunstleri and intermedia was identified to be toxic at 500 and 1000 μg/ml (P<0.001). The insulin secretion for Ficus deltoidea var. deltoidea, angustifolia, and motleyana was dose-dependent; further evaluation suggested that Ficus deltoidea var. trengganuensis was involved in K ᴬᵀᴾ -independent pathway. This study suggests that standardized methanolic extracts of Ficus deltoidea varieties have an insulinotropic effect on clonal BRIN BD11 cell line and can be utilized as a modern candidate of antidiabetic agents targeting the escalation for insulin secretion from pancreatic beta cells. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74701/1/Insulinotropic%20activity.pdf Yahaya, Nurshieren and Mohd Dom, Nur Sumirah and Adam, Zainah and Hamid, Muhajir (2018) Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities. Evidence-based Complementary and Alternative Medicine, 2018. pp. 1-8. ISSN 1741-427X; ESSN: 1741-4288 10.1155/2018/3769874 |
| spellingShingle | Yahaya, Nurshieren Mohd Dom, Nur Sumirah Adam, Zainah Hamid, Muhajir Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title | Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title_full | Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title_fullStr | Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title_full_unstemmed | Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title_short | Insulinotropic activity of standardized methanolic extracts of Ficus deltoidea from seven varities |
| title_sort | insulinotropic activity of standardized methanolic extracts of ficus deltoidea from seven varities |
| url | http://psasir.upm.edu.my/id/eprint/74701/1/Insulinotropic%20activity.pdf |
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