Label-free dengue E protein detection using a functionalized tapered optical fiber sensor
The surge of Dengue cases around the globe has intensified the demand for a reliable diagnostic method. The work demonstrates, to the best of our knowledge, the first label-free optical based sensor for detection of Dengue II E proteins. This was achieved by utilizing tapered optical fiber that has...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74812/1/Label-free%20dengue%20E%20protein%20detection%20using%20a%20functionalized%20tapered%20optical%20fiber%20sensor.pdf |
| _version_ | 1796979765909913600 |
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| author | Mustapha Kamil, Yasmin Abu Bakar, M. H. Mustapa, Mohd Azmir Yaacob, Mohd Hanif Zainol Abidin, Nadiah Husseini Syahir, Amir Lee, Huijing Mahdi, Mohd Adzir |
| author_facet | Mustapha Kamil, Yasmin Abu Bakar, M. H. Mustapa, Mohd Azmir Yaacob, Mohd Hanif Zainol Abidin, Nadiah Husseini Syahir, Amir Lee, Huijing Mahdi, Mohd Adzir |
| author_sort | Mustapha Kamil, Yasmin |
| collection | UPM |
| description | The surge of Dengue cases around the globe has intensified the demand for a reliable diagnostic method. The work demonstrates, to the best of our knowledge, the first label-free optical based sensor for detection of Dengue II E proteins. This was achieved by utilizing tapered optical fiber that has been functionalized with complementary recombinant antibodies. The fundamental concept of the sensor relies on the interaction between strong evanescent waves resulting from the dimensional change of the fiber and immune complex formed on the surface of the fiber when the virus is present. Sensitivity and detection limit values obtained with the sensor setup are 5.02 nm/nM and 1 pM, respectively, with a standard deviation value of no more than ±0.4. The compact and rapid sensor is a viable alternative for label-free and quantitative assessment of the infection, which may assist in providing better clinical management and understanding of the disease. |
| first_indexed | 2024-03-06T10:13:43Z |
| format | Article |
| id | upm.eprints-74812 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:13:43Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-748122019-12-05T06:54:24Z http://psasir.upm.edu.my/id/eprint/74812/ Label-free dengue E protein detection using a functionalized tapered optical fiber sensor Mustapha Kamil, Yasmin Abu Bakar, M. H. Mustapa, Mohd Azmir Yaacob, Mohd Hanif Zainol Abidin, Nadiah Husseini Syahir, Amir Lee, Huijing Mahdi, Mohd Adzir The surge of Dengue cases around the globe has intensified the demand for a reliable diagnostic method. The work demonstrates, to the best of our knowledge, the first label-free optical based sensor for detection of Dengue II E proteins. This was achieved by utilizing tapered optical fiber that has been functionalized with complementary recombinant antibodies. The fundamental concept of the sensor relies on the interaction between strong evanescent waves resulting from the dimensional change of the fiber and immune complex formed on the surface of the fiber when the virus is present. Sensitivity and detection limit values obtained with the sensor setup are 5.02 nm/nM and 1 pM, respectively, with a standard deviation value of no more than ±0.4. The compact and rapid sensor is a viable alternative for label-free and quantitative assessment of the infection, which may assist in providing better clinical management and understanding of the disease. Elsevier 2018-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74812/1/Label-free%20dengue%20E%20protein%20detection%20using%20a%20functionalized%20tapered%20optical%20fiber%20sensor.pdf Mustapha Kamil, Yasmin and Abu Bakar, M. H. and Mustapa, Mohd Azmir and Yaacob, Mohd Hanif and Zainol Abidin, Nadiah Husseini and Syahir, Amir and Lee, Huijing and Mahdi, Mohd Adzir (2018) Label-free dengue E protein detection using a functionalized tapered optical fiber sensor. Sensors and Actuators B: Chemical, 257. 820 - 828. ISSN 0925-4005; ESSN: 1873-3077 https://www.sciencedirect.com/science/article/pii/S0925400517321263 10.1016/j.snb.2017.11.005 |
| spellingShingle | Mustapha Kamil, Yasmin Abu Bakar, M. H. Mustapa, Mohd Azmir Yaacob, Mohd Hanif Zainol Abidin, Nadiah Husseini Syahir, Amir Lee, Huijing Mahdi, Mohd Adzir Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title | Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title_full | Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title_fullStr | Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title_full_unstemmed | Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title_short | Label-free dengue E protein detection using a functionalized tapered optical fiber sensor |
| title_sort | label free dengue e protein detection using a functionalized tapered optical fiber sensor |
| url | http://psasir.upm.edu.my/id/eprint/74812/1/Label-free%20dengue%20E%20protein%20detection%20using%20a%20functionalized%20tapered%20optical%20fiber%20sensor.pdf |
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