Long-term preservation of Leptospira spp.: challenges and prospects
Preservation of leptospiral cultures is tantamount to success in leptospiral diagnostics, research, and development of preventive strategies. Each Leptospira isolate has imperative value not only in disease diagnosis but also in epidemiology, virulence, pathogenesis, and drug development studies. As...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/74857/1/Long-term%20preservation.pdf |
| _version_ | 1825950385477517312 |
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| author | Philip, Noraini Garba, Bashiru Neela, Vasantha Kumari |
| author_facet | Philip, Noraini Garba, Bashiru Neela, Vasantha Kumari |
| author_sort | Philip, Noraini |
| collection | UPM |
| description | Preservation of leptospiral cultures is tantamount to success in leptospiral diagnostics, research, and development of preventive strategies. Each Leptospira isolate has imperative value not only in disease diagnosis but also in epidemiology, virulence, pathogenesis, and drug development studies. As the number of circulating leptospires is continuously increasing and congruent with the importance to retain their original characteristics and properties, an efficient long-term preservation is critically needed to be well-established. However, the preservation of Leptospira is currently characterized by difficulties and conflicting results mainly due to the biological nature of this organism. Hence, this review seeks to describe the efforts in developing efficient preservation methods, to discover the challenges in preserving this organism and to identify the factors that can contribute to an effective long-term preservation of Leptospira. Through the enlightenment of the previous studies, a potentially effective method has been suggested. The article also attempts to evaluate novel strategies used in other industrial and biotechnological preservation efforts and consider their potential application to the conservation of Leptospira spp. |
| first_indexed | 2024-03-06T10:13:47Z |
| format | Article |
| id | upm.eprints-74857 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:13:47Z |
| publishDate | 2018 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-748572020-03-17T08:15:17Z http://psasir.upm.edu.my/id/eprint/74857/ Long-term preservation of Leptospira spp.: challenges and prospects Philip, Noraini Garba, Bashiru Neela, Vasantha Kumari Preservation of leptospiral cultures is tantamount to success in leptospiral diagnostics, research, and development of preventive strategies. Each Leptospira isolate has imperative value not only in disease diagnosis but also in epidemiology, virulence, pathogenesis, and drug development studies. As the number of circulating leptospires is continuously increasing and congruent with the importance to retain their original characteristics and properties, an efficient long-term preservation is critically needed to be well-established. However, the preservation of Leptospira is currently characterized by difficulties and conflicting results mainly due to the biological nature of this organism. Hence, this review seeks to describe the efforts in developing efficient preservation methods, to discover the challenges in preserving this organism and to identify the factors that can contribute to an effective long-term preservation of Leptospira. Through the enlightenment of the previous studies, a potentially effective method has been suggested. The article also attempts to evaluate novel strategies used in other industrial and biotechnological preservation efforts and consider their potential application to the conservation of Leptospira spp. Springer 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74857/1/Long-term%20preservation.pdf Philip, Noraini and Garba, Bashiru and Neela, Vasantha Kumari (2018) Long-term preservation of Leptospira spp.: challenges and prospects. Applied Microbiology and Biotechnology, 102 (2-3). 5427 - 5435. ISSN 0175-7598, ESSN: 1432-0614 10.1007/s00253-018-9047-9 |
| spellingShingle | Philip, Noraini Garba, Bashiru Neela, Vasantha Kumari Long-term preservation of Leptospira spp.: challenges and prospects |
| title | Long-term preservation of Leptospira spp.: challenges and prospects |
| title_full | Long-term preservation of Leptospira spp.: challenges and prospects |
| title_fullStr | Long-term preservation of Leptospira spp.: challenges and prospects |
| title_full_unstemmed | Long-term preservation of Leptospira spp.: challenges and prospects |
| title_short | Long-term preservation of Leptospira spp.: challenges and prospects |
| title_sort | long term preservation of leptospira spp challenges and prospects |
| url | http://psasir.upm.edu.my/id/eprint/74857/1/Long-term%20preservation.pdf |
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