In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia
The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats we...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Medwell Journals
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7493/1/In-house%20rose%20Bengal%20plate%20agglutination%20test%20%28RBPT%29%20for%20a%20rapid%20diagnosis%20of%20Brucellosis%20in%20goats%20in%20Malaysia.pdf |
| _version_ | 1825944217707347968 |
|---|---|
| author | Othman, Shahaza Bejo, Siti Khairani Zakaria, Zunita Bahaman, Abdul Rani |
| author_facet | Othman, Shahaza Bejo, Siti Khairani Zakaria, Zunita Bahaman, Abdul Rani |
| author_sort | Othman, Shahaza |
| collection | UPM |
| description | The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia. |
| first_indexed | 2024-03-06T07:12:24Z |
| format | Article |
| id | upm.eprints-7493 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:12:24Z |
| publishDate | 2009 |
| publisher | Medwell Journals |
| record_format | dspace |
| spelling | upm.eprints-74932020-07-22T02:48:11Z http://psasir.upm.edu.my/id/eprint/7493/ In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia Othman, Shahaza Bejo, Siti Khairani Zakaria, Zunita Bahaman, Abdul Rani The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia. Medwell Journals 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/7493/1/In-house%20rose%20Bengal%20plate%20agglutination%20test%20%28RBPT%29%20for%20a%20rapid%20diagnosis%20of%20Brucellosis%20in%20goats%20in%20Malaysia.pdf Othman, Shahaza and Bejo, Siti Khairani and Zakaria, Zunita and Bahaman, Abdul Rani (2009) In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia. International Journal of Tropical Medicine, 4 (3). pp. 116-118. ISSN 1816-3319; ESSN: 1818-779X http://www.medwelljournals.com/abstract/?doi=ijtmed.2009.116.118 |
| spellingShingle | Othman, Shahaza Bejo, Siti Khairani Zakaria, Zunita Bahaman, Abdul Rani In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title | In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title_full | In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title_fullStr | In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title_full_unstemmed | In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title_short | In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia |
| title_sort | in house rose bengal plate agglutination test rbpt for a rapid diagnosis of brucellosis in goats in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/7493/1/In-house%20rose%20Bengal%20plate%20agglutination%20test%20%28RBPT%29%20for%20a%20rapid%20diagnosis%20of%20Brucellosis%20in%20goats%20in%20Malaysia.pdf |
| work_keys_str_mv | AT othmanshahaza inhouserosebengalplateagglutinationtestrbptforarapiddiagnosisofbrucellosisingoatsinmalaysia AT bejositikhairani inhouserosebengalplateagglutinationtestrbptforarapiddiagnosisofbrucellosisingoatsinmalaysia AT zakariazunita inhouserosebengalplateagglutinationtestrbptforarapiddiagnosisofbrucellosisingoatsinmalaysia AT bahamanabdulrani inhouserosebengalplateagglutinationtestrbptforarapiddiagnosisofbrucellosisingoatsinmalaysia |