Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi
Detection of porcine plasma using indirect ELISA was developed using mAb B4E1 for the prevention of their usage in human food that creates religious and health conflicts. The immunoassay has a CV<20% and did not cross-react to other meat and non-meat proteins. The sensitivity of the assay is 0.25...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2018
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/75024/1/Monoclonal%20antibody-based%20enzyme%20immunoassay%20for%20detection%20of%20porcine%20plasma%20in%20fish%20surimi.pdf |
| _version_ | 1796979789415841792 |
|---|---|
| author | Raja NharI, Raja Mohd Hafidz Khairil Mokhtar, Nur Fadhilah Hanish, Irwan Hamid, Muhajir |
| author_facet | Raja NharI, Raja Mohd Hafidz Khairil Mokhtar, Nur Fadhilah Hanish, Irwan Hamid, Muhajir |
| author_sort | Raja NharI, Raja Mohd Hafidz |
| collection | UPM |
| description | Detection of porcine plasma using indirect ELISA was developed using mAb B4E1 for the prevention of their usage in human food that creates religious and health conflicts. The immunoassay has a CV<20% and did not cross-react to other meat and non-meat proteins. The sensitivity of the assay is 0.25% (w/w) of porcine plasma in spiked raw and cooked fish surimi. The assay did not produce a false positive result for any of the commercial fish surimi tested that were not contain porcine plasma. Determination of a 60-kDa antigenic protein of porcine blood using Western Blot confirmed its presence in the plasma fraction of the porcine blood. Further proteomic analysis involving liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed the 60-kDa protein to be porcine serum albumin. |
| first_indexed | 2024-03-06T10:14:04Z |
| format | Article |
| id | upm.eprints-75024 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:14:04Z |
| publishDate | 2018 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-750242020-04-20T13:52:28Z http://psasir.upm.edu.my/id/eprint/75024/ Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi Raja NharI, Raja Mohd Hafidz Khairil Mokhtar, Nur Fadhilah Hanish, Irwan Hamid, Muhajir Detection of porcine plasma using indirect ELISA was developed using mAb B4E1 for the prevention of their usage in human food that creates religious and health conflicts. The immunoassay has a CV<20% and did not cross-react to other meat and non-meat proteins. The sensitivity of the assay is 0.25% (w/w) of porcine plasma in spiked raw and cooked fish surimi. The assay did not produce a false positive result for any of the commercial fish surimi tested that were not contain porcine plasma. Determination of a 60-kDa antigenic protein of porcine blood using Western Blot confirmed its presence in the plasma fraction of the porcine blood. Further proteomic analysis involving liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed the 60-kDa protein to be porcine serum albumin. Taylor & Francis 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75024/1/Monoclonal%20antibody-based%20enzyme%20immunoassay%20for%20detection%20of%20porcine%20plasma%20in%20fish%20surimi.pdf Raja NharI, Raja Mohd Hafidz and Khairil Mokhtar, Nur Fadhilah and Hanish, Irwan and Hamid, Muhajir (2018) Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment, 35 (5). 807 - 817. ISSN 1944-0049; ESSN: 1944-0057 10.1080/19440049.2017.1420920 |
| spellingShingle | Raja NharI, Raja Mohd Hafidz Khairil Mokhtar, Nur Fadhilah Hanish, Irwan Hamid, Muhajir Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title | Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title_full | Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title_fullStr | Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title_full_unstemmed | Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title_short | Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi |
| title_sort | monoclonal antibody based enzyme immunoassay for detection of porcine plasma in fish surimi |
| url | http://psasir.upm.edu.my/id/eprint/75024/1/Monoclonal%20antibody-based%20enzyme%20immunoassay%20for%20detection%20of%20porcine%20plasma%20in%20fish%20surimi.pdf |
| work_keys_str_mv | AT rajanharirajamohdhafidz monoclonalantibodybasedenzymeimmunoassayfordetectionofporcineplasmainfishsurimi AT khairilmokhtarnurfadhilah monoclonalantibodybasedenzymeimmunoassayfordetectionofporcineplasmainfishsurimi AT hanishirwan monoclonalantibodybasedenzymeimmunoassayfordetectionofporcineplasmainfishsurimi AT hamidmuhajir monoclonalantibodybasedenzymeimmunoassayfordetectionofporcineplasmainfishsurimi |