Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors
Background Retinal degeneration is a condition ensued by various ocular disorders such as artery occlusion, diabetic retinopathy, retrolental fibroplasia and retinitis pigmentosa which cause abnormal loss of photoreceptor cells and lead to eventual vision impairment. No efficient treatment has yet...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75028/1/Morphological%20and.pdf |
| _version_ | 1825950403735322624 |
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| author | Shi, Qiang Alsaeedi, Hiba Amer Cheng, Yuhong Hao, Yang Li, Tong Kumar, Suresh Akon, Higuchi .Alarfaj, Abdullah A Rusheni, Munisvaradass Mok, Pooi Ling Pei, Cheng |
| author_facet | Shi, Qiang Alsaeedi, Hiba Amer Cheng, Yuhong Hao, Yang Li, Tong Kumar, Suresh Akon, Higuchi .Alarfaj, Abdullah A Rusheni, Munisvaradass Mok, Pooi Ling Pei, Cheng |
| author_sort | Shi, Qiang |
| collection | UPM |
| description | Background
Retinal degeneration is a condition ensued by various ocular disorders such as artery occlusion, diabetic retinopathy, retrolental fibroplasia and retinitis pigmentosa which cause abnormal loss of photoreceptor cells and lead to eventual vision impairment. No efficient treatment has yet been found, however, the use of stem cell therapy such as bone marrow and embryonic stem cells has opened a new treatment modality for retinal degenerative diseases. The major goal of this study is to analyze the potential of endothelial progenitor cells derived from bone marrow to differentiate into retinal neural cells for regenerative medicine purposes.
Methods
In this study, endothelial progenitor cells were induced in-vitro with photoreceptor growth factor (taurine) for 21 days. Subsequently, the morphology and gene expression of CRX and RHO of the photoreceptors-induced EPCs were examined through immunostaining assay.
Findings
The results indicated that the induced endothelial progenitor cells demonstrated positive gene expression of CRX and RHO. Our findings suggested that EPC cells may have a high advantage in cell replacement therapy for treating eye disease, in addition to other neural diseases, and may be a suitable cell source in regenerative medicine for eye disorders. |
| first_indexed | 2024-03-06T10:14:04Z |
| format | Article |
| id | upm.eprints-75028 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:14:04Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-750282020-03-17T08:17:26Z http://psasir.upm.edu.my/id/eprint/75028/ Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors Shi, Qiang Alsaeedi, Hiba Amer Cheng, Yuhong Hao, Yang Li, Tong Kumar, Suresh Akon, Higuchi .Alarfaj, Abdullah A Rusheni, Munisvaradass Mok, Pooi Ling Pei, Cheng Background Retinal degeneration is a condition ensued by various ocular disorders such as artery occlusion, diabetic retinopathy, retrolental fibroplasia and retinitis pigmentosa which cause abnormal loss of photoreceptor cells and lead to eventual vision impairment. No efficient treatment has yet been found, however, the use of stem cell therapy such as bone marrow and embryonic stem cells has opened a new treatment modality for retinal degenerative diseases. The major goal of this study is to analyze the potential of endothelial progenitor cells derived from bone marrow to differentiate into retinal neural cells for regenerative medicine purposes. Methods In this study, endothelial progenitor cells were induced in-vitro with photoreceptor growth factor (taurine) for 21 days. Subsequently, the morphology and gene expression of CRX and RHO of the photoreceptors-induced EPCs were examined through immunostaining assay. Findings The results indicated that the induced endothelial progenitor cells demonstrated positive gene expression of CRX and RHO. Our findings suggested that EPC cells may have a high advantage in cell replacement therapy for treating eye disease, in addition to other neural diseases, and may be a suitable cell source in regenerative medicine for eye disorders. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75028/1/Morphological%20and.pdf Shi, Qiang and Alsaeedi, Hiba Amer and Cheng, Yuhong and Hao, Yang and Li, Tong and Kumar, Suresh and Akon, Higuchi and .Alarfaj, Abdullah A and Rusheni, Munisvaradass and Mok, Pooi Ling and Pei, Cheng (2018) Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors. Journal of Photochemistry and Photobiology B: Biology, 183. 127 - 132. ISSN 1011-1344, ESSN: 1873-2682 10.1016/j.jphotobiol.2018.04.003 |
| spellingShingle | Shi, Qiang Alsaeedi, Hiba Amer Cheng, Yuhong Hao, Yang Li, Tong Kumar, Suresh Akon, Higuchi .Alarfaj, Abdullah A Rusheni, Munisvaradass Mok, Pooi Ling Pei, Cheng Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title | Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title_full | Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title_fullStr | Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title_full_unstemmed | Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title_short | Morphological and genetical changes of endothelial progenitor cells afterin-vitroconversion into photoreceptors |
| title_sort | morphological and genetical changes of endothelial progenitor cells afterin vitroconversion into photoreceptors |
| url | http://psasir.upm.edu.my/id/eprint/75028/1/Morphological%20and.pdf |
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