Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana
Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (...
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| Format: | Article |
| Language: | English |
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Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/75167/1/Optimization%20of%20sub-critical%20water%20extraction%20%28SWE%29%20of%20lipid%20and%20eicosapentaenoic%20acid%20%28EPA%29%20from%20Nannochloropsis%20gaditana.pdf |
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| author | Chon, Bernard Han Ho Mustapa Kamal, Siti Mazlina Danquah, Michael K. Harun, Razif |
| author_facet | Chon, Bernard Han Ho Mustapa Kamal, Siti Mazlina Danquah, Michael K. Harun, Razif |
| author_sort | Chon, Bernard Han Ho |
| collection | UPM |
| description | Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50 g algae/L were 18.278 wt% of total biomass and 14.036 wt% of total fatty acid methyl ester (FAME), respectively. |
| first_indexed | 2024-03-06T10:14:21Z |
| format | Article |
| id | upm.eprints-75167 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:14:21Z |
| publishDate | 2018 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | upm.eprints-751672020-07-16T07:08:18Z http://psasir.upm.edu.my/id/eprint/75167/ Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana Chon, Bernard Han Ho Mustapa Kamal, Siti Mazlina Danquah, Michael K. Harun, Razif Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50 g algae/L were 18.278 wt% of total biomass and 14.036 wt% of total fatty acid methyl ester (FAME), respectively. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75167/1/Optimization%20of%20sub-critical%20water%20extraction%20%28SWE%29%20of%20lipid%20and%20eicosapentaenoic%20acid%20%28EPA%29%20from%20Nannochloropsis%20gaditana.pdf Chon, Bernard Han Ho and Mustapa Kamal, Siti Mazlina and Danquah, Michael K. and Harun, Razif (2018) Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana. Biomed Research International, 2018. art. no. 8273581. pp. 1-11. ISSN 2314-6133; ESSN: 2314-6141 http://downloads.hindawi.com/journals/bmri/2018/8273581.pdf 10.1155/2018/8273581 |
| spellingShingle | Chon, Bernard Han Ho Mustapa Kamal, Siti Mazlina Danquah, Michael K. Harun, Razif Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title | Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title_full | Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title_fullStr | Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title_full_unstemmed | Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title_short | Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana |
| title_sort | optimization of sub critical water extraction swe of lipid and eicosapentaenoic acid epa from nannochloropsis gaditana |
| url | http://psasir.upm.edu.my/id/eprint/75167/1/Optimization%20of%20sub-critical%20water%20extraction%20%28SWE%29%20of%20lipid%20and%20eicosapentaenoic%20acid%20%28EPA%29%20from%20Nannochloropsis%20gaditana.pdf |
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