Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2007
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/7521/1/Cardamonin%20inhibits%20COX%20and%20iNOS%20expression%20via%20inhibition%20of%20p65NF.pdf |
| _version_ | 1796967343556919296 |
|---|---|
| author | Israf Ali, Daud Ahmad Tajul Arifin, Khaizurin Ahmad, Syahida Lajis, Md Nordin Shari, Khozirah |
| author_facet | Israf Ali, Daud Ahmad Tajul Arifin, Khaizurin Ahmad, Syahida Lajis, Md Nordin Shari, Khozirah |
| author_sort | Israf Ali, Daud Ahmad |
| collection | UPM |
| description | Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic activity, and key molecules in the NF-κB pathway in order to determine its molecular target. Cardamonin suppressed the production of NO and PGE2 in interferon-γ (IFN-γ)- and lipopolysaccharide (LPS)-induced RAW 264.7 cells. This inhibition was demonstrated to be caused by a dose-dependent down-regulation of both inducible enzymes, iNOS and COX-2, without direct effect upon iNOS or COX-2 enzyme activity. Subsequently we determined that the inhibition of inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation and degradation of I-κBα, which resulted in a reduction of p65NF-κB nuclear translocation. We conclude that cardamonin is a potential anti-inflammatory drug lead that targets the NF-κB pathway.
|
| first_indexed | 2024-03-06T07:12:29Z |
| format | Article |
| id | upm.eprints-7521 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:12:29Z |
| publishDate | 2007 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-75212015-10-01T08:00:17Z http://psasir.upm.edu.my/id/eprint/7521/ Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells Israf Ali, Daud Ahmad Tajul Arifin, Khaizurin Ahmad, Syahida Lajis, Md Nordin Shari, Khozirah Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic activity, and key molecules in the NF-κB pathway in order to determine its molecular target. Cardamonin suppressed the production of NO and PGE2 in interferon-γ (IFN-γ)- and lipopolysaccharide (LPS)-induced RAW 264.7 cells. This inhibition was demonstrated to be caused by a dose-dependent down-regulation of both inducible enzymes, iNOS and COX-2, without direct effect upon iNOS or COX-2 enzyme activity. Subsequently we determined that the inhibition of inducible enzyme expression was due to a dose-dependent inhibition of phosphorylation and degradation of I-κBα, which resulted in a reduction of p65NF-κB nuclear translocation. We conclude that cardamonin is a potential anti-inflammatory drug lead that targets the NF-κB pathway. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7521/1/Cardamonin%20inhibits%20COX%20and%20iNOS%20expression%20via%20inhibition%20of%20p65NF.pdf Israf Ali, Daud Ahmad and Tajul Arifin, Khaizurin and Ahmad, Syahida and Lajis, Md Nordin and Shari, Khozirah (2007) Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells. Molecular Immunology, 44 (5). pp. 673-679. ISSN 0161-5890 http://dx.doi.org/10.1016/j.molimm.2006.04.025 10.1016/j.molimm.2006.04.025 English |
| spellingShingle | Israf Ali, Daud Ahmad Tajul Arifin, Khaizurin Ahmad, Syahida Lajis, Md Nordin Shari, Khozirah Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells |
| title | Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
|
| title_full | Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
|
| title_fullStr | Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
|
| title_full_unstemmed | Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
|
| title_short | Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-κB nuclear translocation and Iκ-B phosphorylation in RAW 264.7 macrophage cells
|
| title_sort | cardamonin inhibits cox and inos expression via inhibition of p65nf κb nuclear translocation and iκ b phosphorylation in raw 264 7 macrophage cells |
| url | http://psasir.upm.edu.my/id/eprint/7521/1/Cardamonin%20inhibits%20COX%20and%20iNOS%20expression%20via%20inhibition%20of%20p65NF.pdf |
| work_keys_str_mv | AT israfalidaudahmad cardamonininhibitscoxandinosexpressionviainhibitionofp65nfkbnucleartranslocationandikbphosphorylationinraw2647macrophagecells AT tajularifinkhaizurin cardamonininhibitscoxandinosexpressionviainhibitionofp65nfkbnucleartranslocationandikbphosphorylationinraw2647macrophagecells AT ahmadsyahida cardamonininhibitscoxandinosexpressionviainhibitionofp65nfkbnucleartranslocationandikbphosphorylationinraw2647macrophagecells AT lajismdnordin cardamonininhibitscoxandinosexpressionviainhibitionofp65nfkbnucleartranslocationandikbphosphorylationinraw2647macrophagecells AT sharikhozirah cardamonininhibitscoxandinosexpressionviainhibitionofp65nfkbnucleartranslocationandikbphosphorylationinraw2647macrophagecells |