Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex
An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilizatio...
| প্রধান লেখক: | , , , , , |
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| বিন্যাস: | Conference or Workshop Item |
| ভাষা: | English |
| প্রকাশিত: |
Institute of Research Engineers and Doctors, USA
2015
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| অনলাইন ব্যবহার করুন: | http://psasir.upm.edu.my/id/eprint/75445/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides.pdf |
| _version_ | 1825950467769761792 |
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| author | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain |
| author_facet | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain |
| author_sort | Abdullah Halid, Nurul Izni |
| collection | UPM |
| description | An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM. |
| first_indexed | 2024-03-06T10:15:03Z |
| format | Conference or Workshop Item |
| id | upm.eprints-75445 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:15:03Z |
| publishDate | 2015 |
| publisher | Institute of Research Engineers and Doctors, USA |
| record_format | dspace |
| spelling | upm.eprints-754452019-10-07T04:13:57Z http://psasir.upm.edu.my/id/eprint/75445/ Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM. Institute of Research Engineers and Doctors, USA 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/75445/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides.pdf Abdullah Halid, Nurul Izni and Hasbullah, Siti Aishah and Ahmad, Haslina and Lee, Yook Heng and Abd Karim, Nurul Huda and Harun, Siti Norain (2015) Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex. In: Third International Conference on Advances in Bio-Informatics, Bio-Technology and Environmental Engineering (ABBE 2015), 26-27 May 2015, Birmingham, United Kingdom. (pp. 15-17). https://www.seekdl.org/conferences/paper/details/6076.html 10.15224/978-1-63248-060-6-04 |
| spellingShingle | Abdullah Halid, Nurul Izni Hasbullah, Siti Aishah Ahmad, Haslina Lee, Yook Heng Abd Karim, Nurul Huda Harun, Siti Norain Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title | Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title_full | Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title_fullStr | Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title_full_unstemmed | Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title_short | Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex |
| title_sort | electrochemical dna biosensor for detection of porcine oligonucleotides using ru bpy 2pip 2 complex |
| url | http://psasir.upm.edu.my/id/eprint/75445/1/Electrochemical%20DNA%20biosensor%20for%20detection%20of%20porcine%20oligonucleotides.pdf |
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