Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?

Pseudogene considered as non functional gene in many organisms. Based on the study, there are several pseodugenes embaded in Escherichia coli (E.coli) genome. yqiG is one of the pseudogenes found in E.coli.. Hydorgen is by-product produces during the degradation of glucose through glyclysis pathway....

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Main Authors: Zakaria, Muhammad Azman, Mohd Yusoff, Mohd Zulkhairi, Hassan, Mohd Ali, Zakaria @ Mamat, Mohd Rafein, Maeda, Toshinari
格式: Conference or Workshop Item
语言:English
出版: Faculty of Computer Science and Information Technology, Universiti Putra Malaysia 2015
在线阅读:http://psasir.upm.edu.my/id/eprint/77194/1/saes2015-48.pdf
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author Zakaria, Muhammad Azman
Mohd Yusoff, Mohd Zulkhairi
Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Maeda, Toshinari
author_facet Zakaria, Muhammad Azman
Mohd Yusoff, Mohd Zulkhairi
Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Maeda, Toshinari
author_sort Zakaria, Muhammad Azman
collection UPM
description Pseudogene considered as non functional gene in many organisms. Based on the study, there are several pseodugenes embaded in Escherichia coli (E.coli) genome. yqiG is one of the pseudogenes found in E.coli.. Hydorgen is by-product produces during the degradation of glucose through glyclysis pathway. Theoritically, one mole of glucose may produces two mole of format and finally produce two mol of hydrogen. In this study, deletion of yqiG in E.coli has shown hydrogen deficiency during fermentation of glucose as carbon source. However, an opposite effect has observed from medium consist of formate as its substrate. Longer lag phase observed in formate medium during hydrogen production as the absence of YqiG. The result has confirmed that YqiG has a role during hydrogen production. It is probably, controls the gene expression and may helps on the maturation process of protein. Hence, YqiG is not a junk gene instead it is one of the essential proteins during hydrogen metabolism of E.coli.
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format Conference or Workshop Item
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institution Universiti Putra Malaysia
language English
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spelling upm.eprints-771942020-03-04T06:57:26Z http://psasir.upm.edu.my/id/eprint/77194/ Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production? Zakaria, Muhammad Azman Mohd Yusoff, Mohd Zulkhairi Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Maeda, Toshinari Pseudogene considered as non functional gene in many organisms. Based on the study, there are several pseodugenes embaded in Escherichia coli (E.coli) genome. yqiG is one of the pseudogenes found in E.coli.. Hydorgen is by-product produces during the degradation of glucose through glyclysis pathway. Theoritically, one mole of glucose may produces two mole of format and finally produce two mol of hydrogen. In this study, deletion of yqiG in E.coli has shown hydrogen deficiency during fermentation of glucose as carbon source. However, an opposite effect has observed from medium consist of formate as its substrate. Longer lag phase observed in formate medium during hydrogen production as the absence of YqiG. The result has confirmed that YqiG has a role during hydrogen production. It is probably, controls the gene expression and may helps on the maturation process of protein. Hence, YqiG is not a junk gene instead it is one of the essential proteins during hydrogen metabolism of E.coli. Faculty of Computer Science and Information Technology, Universiti Putra Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77194/1/saes2015-48.pdf Zakaria, Muhammad Azman and Mohd Yusoff, Mohd Zulkhairi and Hassan, Mohd Ali and Zakaria @ Mamat, Mohd Rafein and Maeda, Toshinari (2015) Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production? In: 3rd International Symposium on Applied Engineering and Sciences (SAES2015), 23-24 Nov. 2015, Universiti Putra Malaysia. (pp. 283-285).
spellingShingle Zakaria, Muhammad Azman
Mohd Yusoff, Mohd Zulkhairi
Hassan, Mohd Ali
Zakaria @ Mamat, Mohd Rafein
Maeda, Toshinari
Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title_full Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title_fullStr Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title_full_unstemmed Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title_short Escherichia coli yqiG: pseudogene or functional allele for biohydrogen production?
title_sort escherichia coli yqig pseudogene or functional allele for biohydrogen production
url http://psasir.upm.edu.my/id/eprint/77194/1/saes2015-48.pdf
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AT hassanmohdali escherichiacoliyqigpseudogeneorfunctionalalleleforbiohydrogenproduction
AT zakariamamatmohdrafein escherichiacoliyqigpseudogeneorfunctionalalleleforbiohydrogenproduction
AT maedatoshinari escherichiacoliyqigpseudogeneorfunctionalalleleforbiohydrogenproduction