Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals.
Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed usi...
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| Format: | Article |
| Language: | English English English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7742/1/Application%20of%20MPN.pdf http://psasir.upm.edu.my/id/eprint/7742/2/Application%20of%20MPN-PCR%20in%20biosafety%20of%20Bacillus%20cereus%20s.l.%20for%20ready-to-eat%20cereals.pdf |
| _version_ | 1825944265833840640 |
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| author | Lee, Hai Yen Chai, Lay Ching Tang, Sui Yan Selamat, Jinap Mohammad Ghazali, Farinazleen Radu, Son |
| author_facet | Lee, Hai Yen Chai, Lay Ching Tang, Sui Yan Selamat, Jinap Mohammad Ghazali, Farinazleen Radu, Son |
| author_sort | Lee, Hai Yen |
| collection | UPM |
| description | Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed using MPN-PCR method. Results showed that 78% of the processed cereal products intended for direct consumption were positive for the presence of B. cereus with concentrations ranging from as low as 30 MPN/g to more than 24,000 MPN/g. The concentration obtained from this study also reflects on the differences in the contamination level between the infant food, raw cereals, cereal bars, ready-to-eat breakfast cereals and pre-mixed drinks examined. Hence, application of the MPN-PCR method was found to be useful to address the biosafety concerns of B. cereus in ready-to-eat cereals. |
| first_indexed | 2024-03-06T07:13:08Z |
| format | Article |
| id | upm.eprints-7742 |
| institution | Universiti Putra Malaysia |
| language | English English English |
| last_indexed | 2024-03-06T07:13:08Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-77422015-10-05T05:58:21Z http://psasir.upm.edu.my/id/eprint/7742/ Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. Lee, Hai Yen Chai, Lay Ching Tang, Sui Yan Selamat, Jinap Mohammad Ghazali, Farinazleen Radu, Son Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed using MPN-PCR method. Results showed that 78% of the processed cereal products intended for direct consumption were positive for the presence of B. cereus with concentrations ranging from as low as 30 MPN/g to more than 24,000 MPN/g. The concentration obtained from this study also reflects on the differences in the contamination level between the infant food, raw cereals, cereal bars, ready-to-eat breakfast cereals and pre-mixed drinks examined. Hence, application of the MPN-PCR method was found to be useful to address the biosafety concerns of B. cereus in ready-to-eat cereals. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7742/1/Application%20of%20MPN.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/7742/2/Application%20of%20MPN-PCR%20in%20biosafety%20of%20Bacillus%20cereus%20s.l.%20for%20ready-to-eat%20cereals.pdf Lee, Hai Yen and Chai, Lay Ching and Tang, Sui Yan and Selamat, Jinap and Mohammad Ghazali, Farinazleen and Radu, Son (2009) Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. Food Control, 20 (11). pp. 1068-1071. ISSN 0956-7135 http://dx.doi.org/10.1016/j.foodcont.2009.01.009 10.1016/j.foodcont.2009.01.009 English |
| spellingShingle | Lee, Hai Yen Chai, Lay Ching Tang, Sui Yan Selamat, Jinap Mohammad Ghazali, Farinazleen Radu, Son Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title | Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title_full | Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title_fullStr | Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title_full_unstemmed | Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title_short | Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. |
| title_sort | application of mpn pcr in biosafety of bacillus cereus s l for ready to eat cereals |
| url | http://psasir.upm.edu.my/id/eprint/7742/1/Application%20of%20MPN.pdf http://psasir.upm.edu.my/id/eprint/7742/2/Application%20of%20MPN-PCR%20in%20biosafety%20of%20Bacillus%20cereus%20s.l.%20for%20ready-to-eat%20cereals.pdf |
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