Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1
Although more than 100 genome sequences of Pasteurella multocida are available, comprehensive and complete genome sequence analysis is limited. This study describes the analysis of complete genome sequence and pathogenomics of P. multocida strain PMTB2.1. The genome of PMTB2.1 has 2176 genes with mo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77879/1/77879.pdf |
| _version_ | 1796980373931950080 |
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| author | Jabeen, Shagufta Yap, Huan Yong Abdullah, Faez Firdaus Jesse Zakaria, Zunita Mat Isa, Nurulfiza Tan, Yung Chie Yap, Soon Joo Satharasinghe, Dilan Amila Omar, Abdul Rahman |
| author_facet | Jabeen, Shagufta Yap, Huan Yong Abdullah, Faez Firdaus Jesse Zakaria, Zunita Mat Isa, Nurulfiza Tan, Yung Chie Yap, Soon Joo Satharasinghe, Dilan Amila Omar, Abdul Rahman |
| author_sort | Jabeen, Shagufta |
| collection | UPM |
| description | Although more than 100 genome sequences of Pasteurella multocida are available, comprehensive and complete genome sequence analysis is limited. This study describes the analysis of complete genome sequence and pathogenomics of P. multocida strain PMTB2.1. The genome of PMTB2.1 has 2176 genes with more than 40 coding sequences associated with iron regulation and 140 virulence genes including the complete tad locus. The tad locus includes several previously uncharacterized genes such as flp2, rcpC and tadV genes. A transposable phage resembling to Mu phages was identified in P. multocida that has not been identified in any other serotype yet. The multi-locus sequence typing analysis assigned the PMTB2.1 genome sequence as type ST101, while the comparative genome analysis showed that PMTB2.1 is closely related to other P. multocida strains with the genomic distance of less than 0.13. The expression profiling of iron regulating-genes of PMTB2.1 was characterized under iron-limited environment. Results showed significant changes in the expression profiles of iron-regulating genes (p < 0.05) whereas the highest expression of fecE gene (281 fold) at 30 min suggests utilization of the outer-membrane proteins system in iron acquisition at an early stage of growth. This study showed the phylogenomic relatedness of P. multocida and improved annotation of important genes and functional characterization of iron-regulating genes of importance to the bacterial growth. |
| first_indexed | 2024-03-06T10:22:05Z |
| format | Article |
| id | upm.eprints-77879 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:22:05Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-778792020-05-04T17:36:20Z http://psasir.upm.edu.my/id/eprint/77879/ Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 Jabeen, Shagufta Yap, Huan Yong Abdullah, Faez Firdaus Jesse Zakaria, Zunita Mat Isa, Nurulfiza Tan, Yung Chie Yap, Soon Joo Satharasinghe, Dilan Amila Omar, Abdul Rahman Although more than 100 genome sequences of Pasteurella multocida are available, comprehensive and complete genome sequence analysis is limited. This study describes the analysis of complete genome sequence and pathogenomics of P. multocida strain PMTB2.1. The genome of PMTB2.1 has 2176 genes with more than 40 coding sequences associated with iron regulation and 140 virulence genes including the complete tad locus. The tad locus includes several previously uncharacterized genes such as flp2, rcpC and tadV genes. A transposable phage resembling to Mu phages was identified in P. multocida that has not been identified in any other serotype yet. The multi-locus sequence typing analysis assigned the PMTB2.1 genome sequence as type ST101, while the comparative genome analysis showed that PMTB2.1 is closely related to other P. multocida strains with the genomic distance of less than 0.13. The expression profiling of iron regulating-genes of PMTB2.1 was characterized under iron-limited environment. Results showed significant changes in the expression profiles of iron-regulating genes (p < 0.05) whereas the highest expression of fecE gene (281 fold) at 30 min suggests utilization of the outer-membrane proteins system in iron acquisition at an early stage of growth. This study showed the phylogenomic relatedness of P. multocida and improved annotation of important genes and functional characterization of iron-regulating genes of importance to the bacterial growth. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77879/1/77879.pdf Jabeen, Shagufta and Yap, Huan Yong and Abdullah, Faez Firdaus Jesse and Zakaria, Zunita and Mat Isa, Nurulfiza and Tan, Yung Chie and Yap, Soon Joo and Satharasinghe, Dilan Amila and Omar, Abdul Rahman (2019) Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1. Genes, 10 (2). art. no. 81. pp. 1-21. ISSN 2073-4425 https://www.mdpi.com/2073-4425/10/2/81 10.3390/genes10020081 |
| spellingShingle | Jabeen, Shagufta Yap, Huan Yong Abdullah, Faez Firdaus Jesse Zakaria, Zunita Mat Isa, Nurulfiza Tan, Yung Chie Yap, Soon Joo Satharasinghe, Dilan Amila Omar, Abdul Rahman Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title | Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title_full | Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title_fullStr | Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title_full_unstemmed | Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title_short | Complete genome sequence analysis and characterization of selected iron regulation genes of Pasteurella multocida serotype A strain PMTB2.1 |
| title_sort | complete genome sequence analysis and characterization of selected iron regulation genes of pasteurella multocida serotype a strain pmtb2 1 |
| url | http://psasir.upm.edu.my/id/eprint/77879/1/77879.pdf |
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