Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77882/1/77882.pdf |
| _version_ | 1796980374542221312 |
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| author | Lasano, Nur Fatimah Hamid, Azizah Karim, Roselina Pak Dek, Mohd Sabri Shukri, Radhiah Ramli, Nurul Shazini |
| author_facet | Lasano, Nur Fatimah Hamid, Azizah Karim, Roselina Pak Dek, Mohd Sabri Shukri, Radhiah Ramli, Nurul Shazini |
| author_sort | Lasano, Nur Fatimah |
| collection | UPM |
| description | Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry. |
| first_indexed | 2024-03-06T10:22:05Z |
| format | Article |
| id | upm.eprints-77882 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:22:05Z |
| publishDate | 2019 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-778822020-05-04T17:36:38Z http://psasir.upm.edu.my/id/eprint/77882/ Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel Lasano, Nur Fatimah Hamid, Azizah Karim, Roselina Pak Dek, Mohd Sabri Shukri, Radhiah Ramli, Nurul Shazini Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77882/1/77882.pdf Lasano, Nur Fatimah and Hamid, Azizah and Karim, Roselina and Pak Dek, Mohd Sabri and Shukri, Radhiah and Ramli, Nurul Shazini (2019) Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel. Molecules, 24 (2). art. no. 320. pp. 1-20. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/2/320 10.3390/molecules24020320 |
| spellingShingle | Lasano, Nur Fatimah Hamid, Azizah Karim, Roselina Pak Dek, Mohd Sabri Shukri, Radhiah Ramli, Nurul Shazini Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title | Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title_full | Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title_fullStr | Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title_full_unstemmed | Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title_short | Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel |
| title_sort | nutritional composition anti diabetic properties and identification of active compounds using uhplc esi orbitrap ms ms in mangifera odorata l peel and seed kernel |
| url | http://psasir.upm.edu.my/id/eprint/77882/1/77882.pdf |
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