Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions
Preliminary investigations were carried out to evaluate the antidiabetic effects of the leaves of O. stamineus extracted serially with solvents of increasing polarity (petroleum ether, chloroform, methanol and water); bioassay-guided purification of plant extracts using the subcutaneous glucose tole...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/77967/1/77967.pdf |
| _version_ | 1825950943270666240 |
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| author | Mohamed, Elsnoussi Ali Hussin Mohamed, Ali Jimale Asmawi, Mohd Zaini Sadikun, Amirin Ebrika, Omar Saad Yam, Mun Fei |
| author_facet | Mohamed, Elsnoussi Ali Hussin Mohamed, Ali Jimale Asmawi, Mohd Zaini Sadikun, Amirin Ebrika, Omar Saad Yam, Mun Fei |
| author_sort | Mohamed, Elsnoussi Ali Hussin |
| collection | UPM |
| description | Preliminary investigations were carried out to evaluate the antidiabetic effects of the leaves of O. stamineus extracted serially with solvents of increasing polarity (petroleum ether, chloroform, methanol and water); bioassay-guided purification of plant extracts using the subcutaneous glucose tolerance test (SbGTT) was also carried out. Only the chloroform extract, given at 1 g/kg body weight (b.w.), significantly reduced (P < 0.05) the blood glucose level of rats loaded subcutaneously with 150 mg/kg (b.w.) glucose. The active chloroform extract of O. stamineus was separated into five fractions using a dry flash column chromatography method. Out of the five fractions tested, only chloroform fraction 2 (Cƒ2), at the dose of 1 g/kg (b.w.) significantly inhibited (P < 0.05) blood glucose levels in SbGTT. Active Cƒ2 was split into two sub-fractions Cƒ2-A and Cƒ2-B, using a dry flash column chromatography method. The activities Cƒ2-A and Cƒ2-B were investigated using SbGTT, and the active sub-fraction was then further studied for anti-diabetic effects in a streptozotocin-induced diabetic rat model. The results clearly indicate that Cƒ2-B fraction exhibited a blood glucose lowering effect in fasted treated normal rats after glucose-loading of 150 mg/kg (b.w.). In the acute streptozotocin-induced diabetic rat model, Cƒ2-B did not exhibit a hypoglycemic effect on blood glucose levels up to 7 hours after treatment. Thus, it appears that Cƒ2-B functions similarly to metformin, which has no hypoglycemic effect but demonstrates an antihyperglycemic effect only in normogycemic models. The effect of Cƒ2-B may have no direct stimulatory effects on insulin secretion or on blood glucose levels in diabetic animal models. Verification of the active compound(s) within the active fraction (Cƒ2-B) indicated the presence of terpenoids and, flavonoids, including sinensitin. |
| first_indexed | 2024-03-06T10:22:20Z |
| format | Article |
| id | upm.eprints-77967 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:22:20Z |
| publishDate | 2011 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-779672020-05-04T17:54:45Z http://psasir.upm.edu.my/id/eprint/77967/ Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions Mohamed, Elsnoussi Ali Hussin Mohamed, Ali Jimale Asmawi, Mohd Zaini Sadikun, Amirin Ebrika, Omar Saad Yam, Mun Fei Preliminary investigations were carried out to evaluate the antidiabetic effects of the leaves of O. stamineus extracted serially with solvents of increasing polarity (petroleum ether, chloroform, methanol and water); bioassay-guided purification of plant extracts using the subcutaneous glucose tolerance test (SbGTT) was also carried out. Only the chloroform extract, given at 1 g/kg body weight (b.w.), significantly reduced (P < 0.05) the blood glucose level of rats loaded subcutaneously with 150 mg/kg (b.w.) glucose. The active chloroform extract of O. stamineus was separated into five fractions using a dry flash column chromatography method. Out of the five fractions tested, only chloroform fraction 2 (Cƒ2), at the dose of 1 g/kg (b.w.) significantly inhibited (P < 0.05) blood glucose levels in SbGTT. Active Cƒ2 was split into two sub-fractions Cƒ2-A and Cƒ2-B, using a dry flash column chromatography method. The activities Cƒ2-A and Cƒ2-B were investigated using SbGTT, and the active sub-fraction was then further studied for anti-diabetic effects in a streptozotocin-induced diabetic rat model. The results clearly indicate that Cƒ2-B fraction exhibited a blood glucose lowering effect in fasted treated normal rats after glucose-loading of 150 mg/kg (b.w.). In the acute streptozotocin-induced diabetic rat model, Cƒ2-B did not exhibit a hypoglycemic effect on blood glucose levels up to 7 hours after treatment. Thus, it appears that Cƒ2-B functions similarly to metformin, which has no hypoglycemic effect but demonstrates an antihyperglycemic effect only in normogycemic models. The effect of Cƒ2-B may have no direct stimulatory effects on insulin secretion or on blood glucose levels in diabetic animal models. Verification of the active compound(s) within the active fraction (Cƒ2-B) indicated the presence of terpenoids and, flavonoids, including sinensitin. MDPI 2011 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/77967/1/77967.pdf Mohamed, Elsnoussi Ali Hussin and Mohamed, Ali Jimale and Asmawi, Mohd Zaini and Sadikun, Amirin and Ebrika, Omar Saad and Yam, Mun Fei (2011) Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions. Molecules, 16 (5). pp. 3787-3801. ISSN 1420-3049 https://www.mdpi.com/1420-3049/16/5/3787 10.3390/molecules16053787 |
| spellingShingle | Mohamed, Elsnoussi Ali Hussin Mohamed, Ali Jimale Asmawi, Mohd Zaini Sadikun, Amirin Ebrika, Omar Saad Yam, Mun Fei Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title | Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title_full | Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title_fullStr | Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title_full_unstemmed | Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title_short | Antihyperglycemic effect of Orthosiphon stamineus Benth leaves extract and its bioassay-guided fractions |
| title_sort | antihyperglycemic effect of orthosiphon stamineus benth leaves extract and its bioassay guided fractions |
| url | http://psasir.upm.edu.my/id/eprint/77967/1/77967.pdf |
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