Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel
Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltode...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2012
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/78008/1/78008.pdf |
| _version_ | 1796980400605626368 |
|---|---|
| author | Amid, Mehrnoush Mustafa, Shuhaimi Abd Manap, Mohd Yazid |
| author_facet | Amid, Mehrnoush Mustafa, Shuhaimi Abd Manap, Mohd Yazid |
| author_sort | Amid, Mehrnoush |
| collection | UPM |
| description | Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. |
| first_indexed | 2024-03-06T10:22:28Z |
| format | Article |
| id | upm.eprints-78008 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:22:28Z |
| publishDate | 2012 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-780082020-06-01T09:04:31Z http://psasir.upm.edu.my/id/eprint/78008/ Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel Amid, Mehrnoush Mustafa, Shuhaimi Abd Manap, Mohd Yazid Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. MDPI 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/78008/1/78008.pdf Amid, Mehrnoush and Mustafa, Shuhaimi and Abd Manap, Mohd Yazid (2012) Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. International Journal of Molecular Sciences, 13 (3). pp. 2939-2950. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/13/3/2939 10.3390/ijms13032939 |
| spellingShingle | Amid, Mehrnoush Mustafa, Shuhaimi Abd Manap, Mohd Yazid Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title | Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title_full | Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title_fullStr | Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title_full_unstemmed | Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title_short | Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel |
| title_sort | optimization of freeze drying conditions for purified pectinase from mango mangifera indica cv chokanan peel |
| url | http://psasir.upm.edu.my/id/eprint/78008/1/78008.pdf |
| work_keys_str_mv | AT amidmehrnoush optimizationoffreezedryingconditionsforpurifiedpectinasefrommangomangiferaindicacvchokananpeel AT mustafashuhaimi optimizationoffreezedryingconditionsforpurifiedpectinasefrommangomangiferaindicacvchokananpeel AT abdmanapmohdyazid optimizationoffreezedryingconditionsforpurifiedpectinasefrommangomangiferaindicacvchokananpeel |