| Summary: | Mycobacterium avium subspecies paratuberculosis (MAP) is the aetiology
agent causes Johne‟s disease which viewed as a chronic, contagious bacterial of
intestinal tract in ruminant species. The infection that caused substantial losses to the
farmer was characterized by chronic or intermittent diarrhoea, progressive weight
loss, and decreased production. This study was conducted to determine the presence
of MAP antigen and antibodies in the faeces and serum, respectively, in the cattle. A
total of 213 faecal samples and 71 serum samples were collected from 71 cattle at
Taman Pertanian Universiti (TPU), Universiti Putra Malaysia (UPM). These samples were tested using Ziehl-Neelsen acid-fast stain method for antigen detection and
complement fixation test (CFT) for antibodies detection. Ziehl-Neelsen staining
revealed that 60 (28.2%) of 213 samples were positive for M. avium antigen while,
CFT revealed 3 (4.2%) of 71 serum samples were positive for the antibody.
However, MAP is not the only acid fast organism; thus the determination of species
of the organism should supported by faecal culture. In conclusion, this study showed
that there is MAP infection in the cattle herd at TPU, UPM, with the presence of
antigen causing agent and antibodies in faecal and serum samples, respectively, of cattle.
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