Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens
Pasteurella multocida is the causative agent of fowl cholera in chickens. It causes economic losses to the poultry industry associated with high morbidity and mortality. The objective of this study was to determine the efficacy of inactivated P. multocida either as single or combination of ser...
| Main Author: | |
|---|---|
| Format: | Project Paper Report |
| Language: | English |
| Published: |
2016
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/78178/1/FPV%202016%2019%20-%20IR.pdf |
| _version_ | 1796980435821002752 |
|---|---|
| author | Koh, Sien Ling |
| author_facet | Koh, Sien Ling |
| author_sort | Koh, Sien Ling |
| collection | UPM |
| description | Pasteurella multocida is the causative agent of fowl cholera in chickens. It
causes economic losses to the poultry industry associated with high morbidity and
mortality. The objective of this study was to determine the efficacy of inactivated P.
multocida either as single or combination of serogroup A and D against the bacterial
infection in broiler chickens. Eighty-four, day-old boiler chicken were separated
equally into seven groups. On Day 1, chickens from groups 1 and 4 were inoculated
with serogroup A, groups 2 and 5 with serogroup D while groups 3 and 6 with
combination serogroup A and D. All the chickens were inoculated subcutaneously with 0.1mL of 1X1011 cfu/mL of inactivated P. multocida, except group 7 as control
group. On Day 14, booster was given to groups 4, 5 and 6. On Day 28, all the
chickens were divided into three groups namely non-challenge and challenge either
via intramuscular or intranasal route. The study showed that one chicken each from
group 1 and 7 dead at day 1 and 2 post challenge, respectively. At day 8 post
challenge, all the chickens were scarified. P. multocida was isolated from the dead
chicken in group 1 and 7. P. multocida was not isolated from all the other chickens
scarified at day 8 post challenge. Gross lesions for the dead chickens reveled
generalized congestion in the liver and kidneys while no significant gross lesion
seen in sacrificed chickens. Histopathology findings for the dead chickens reveled
severe congestion with severe necrosis and degeneration of hepatocytes. Severe
pneumonia and severe congestion and necrosis of lung parenchyma were also
recorded. In contrast, mild hepatitis with mild necrosis and degeneration of
hepatocytes and mild pneumonia with mild congestion, degeneration and necrosis at
the lung parenchyma were recorded in all the scarified chickens in all group. In
conclusion, inactivated P. multocida combination of serogroup A and D could
provide better protection against P. multocida serogroup A infection when compared
to the single serogroup A or D. |
| first_indexed | 2024-03-06T10:22:58Z |
| format | Project Paper Report |
| id | upm.eprints-78178 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:22:58Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | upm.eprints-781782020-06-01T02:26:48Z http://psasir.upm.edu.my/id/eprint/78178/ Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens Koh, Sien Ling Pasteurella multocida is the causative agent of fowl cholera in chickens. It causes economic losses to the poultry industry associated with high morbidity and mortality. The objective of this study was to determine the efficacy of inactivated P. multocida either as single or combination of serogroup A and D against the bacterial infection in broiler chickens. Eighty-four, day-old boiler chicken were separated equally into seven groups. On Day 1, chickens from groups 1 and 4 were inoculated with serogroup A, groups 2 and 5 with serogroup D while groups 3 and 6 with combination serogroup A and D. All the chickens were inoculated subcutaneously with 0.1mL of 1X1011 cfu/mL of inactivated P. multocida, except group 7 as control group. On Day 14, booster was given to groups 4, 5 and 6. On Day 28, all the chickens were divided into three groups namely non-challenge and challenge either via intramuscular or intranasal route. The study showed that one chicken each from group 1 and 7 dead at day 1 and 2 post challenge, respectively. At day 8 post challenge, all the chickens were scarified. P. multocida was isolated from the dead chicken in group 1 and 7. P. multocida was not isolated from all the other chickens scarified at day 8 post challenge. Gross lesions for the dead chickens reveled generalized congestion in the liver and kidneys while no significant gross lesion seen in sacrificed chickens. Histopathology findings for the dead chickens reveled severe congestion with severe necrosis and degeneration of hepatocytes. Severe pneumonia and severe congestion and necrosis of lung parenchyma were also recorded. In contrast, mild hepatitis with mild necrosis and degeneration of hepatocytes and mild pneumonia with mild congestion, degeneration and necrosis at the lung parenchyma were recorded in all the scarified chickens in all group. In conclusion, inactivated P. multocida combination of serogroup A and D could provide better protection against P. multocida serogroup A infection when compared to the single serogroup A or D. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78178/1/FPV%202016%2019%20-%20IR.pdf Koh, Sien Ling (2016) Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens. [Project Paper Report] |
| spellingShingle | Koh, Sien Ling Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title | Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title_full | Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title_fullStr | Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title_full_unstemmed | Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title_short | Efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| title_sort | efficacy of inactivated pasteurella multocida against the bacterial infections in broiler chickens |
| url | http://psasir.upm.edu.my/id/eprint/78178/1/FPV%202016%2019%20-%20IR.pdf |
| work_keys_str_mv | AT kohsienling efficacyofinactivatedpasteurellamultocidaagainstthebacterialinfectionsinbroilerchickens |