Effects of reproductive stages on the prevalence of the normal flora in the doe

The genital tract of animals is known to harbour non-specific bacteria known as ῝normal flora῝ but under certain circumstances they can be opportunistic pathogen. Generally, variety of bacteria have been isolated but their role and mechanism are not well understood. This study was designed to...

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Main Author: Ajaman, Norazmanita Edayu
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/78226/1/FPV%202016%2094%20-%20IR.pdf
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author Ajaman, Norazmanita Edayu
author_facet Ajaman, Norazmanita Edayu
author_sort Ajaman, Norazmanita Edayu
collection UPM
description The genital tract of animals is known to harbour non-specific bacteria known as ῝normal flora῝ but under certain circumstances they can be opportunistic pathogen. Generally, variety of bacteria have been isolated but their role and mechanism are not well understood. This study was designed to determine the prevalence of normal bacteria in the vaginal of pregnant and non-pregnant doe with the aims to isolate and identify those bacteria and relate with the different stages of reproduction. For this purpose, a number of fourteen sterile vaginal swabs were collected from 2 groups of doe; non pregnant and pregnant doe (n = 7). The swabs were cultured and incubated for 24h and morphology of the colonies were recorded. Subculture was done to obtain the pure colonies. Gram staining was performed followed by biochemical test accordingly. Bacteria isolated from the vagina of the pregnant group showed higher percentage (55.6%) than non-pregnant group (44.4%). The most dominant bacteria belong to the Stapylococci (58.33%) followed by Streptococci and Enterococcus (8.33%) and other species were isolated at relatively lower rates in both groups. This study showed that hormonal changes during different stages of reproduction had effect on the vaginal bacterial population.
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spelling upm.eprints-782262020-06-05T08:48:01Z http://psasir.upm.edu.my/id/eprint/78226/ Effects of reproductive stages on the prevalence of the normal flora in the doe Ajaman, Norazmanita Edayu The genital tract of animals is known to harbour non-specific bacteria known as ῝normal flora῝ but under certain circumstances they can be opportunistic pathogen. Generally, variety of bacteria have been isolated but their role and mechanism are not well understood. This study was designed to determine the prevalence of normal bacteria in the vaginal of pregnant and non-pregnant doe with the aims to isolate and identify those bacteria and relate with the different stages of reproduction. For this purpose, a number of fourteen sterile vaginal swabs were collected from 2 groups of doe; non pregnant and pregnant doe (n = 7). The swabs were cultured and incubated for 24h and morphology of the colonies were recorded. Subculture was done to obtain the pure colonies. Gram staining was performed followed by biochemical test accordingly. Bacteria isolated from the vagina of the pregnant group showed higher percentage (55.6%) than non-pregnant group (44.4%). The most dominant bacteria belong to the Stapylococci (58.33%) followed by Streptococci and Enterococcus (8.33%) and other species were isolated at relatively lower rates in both groups. This study showed that hormonal changes during different stages of reproduction had effect on the vaginal bacterial population. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78226/1/FPV%202016%2094%20-%20IR.pdf Ajaman, Norazmanita Edayu (2016) Effects of reproductive stages on the prevalence of the normal flora in the doe. [Project Paper Report]
spellingShingle Ajaman, Norazmanita Edayu
Effects of reproductive stages on the prevalence of the normal flora in the doe
title Effects of reproductive stages on the prevalence of the normal flora in the doe
title_full Effects of reproductive stages on the prevalence of the normal flora in the doe
title_fullStr Effects of reproductive stages on the prevalence of the normal flora in the doe
title_full_unstemmed Effects of reproductive stages on the prevalence of the normal flora in the doe
title_short Effects of reproductive stages on the prevalence of the normal flora in the doe
title_sort effects of reproductive stages on the prevalence of the normal flora in the doe
url http://psasir.upm.edu.my/id/eprint/78226/1/FPV%202016%2094%20-%20IR.pdf
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