Effect of sugars on characteristics of Boer goat semen after cryopreservation
In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7888/1/Effect%20of%20sugars%20on%20characteristics%20of%20Boer%20goat%20semen%20after%20cryopreservation.pdf |
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| author | Naing, Soe Win Haron, Abd Wahid Goriman Khan, Mohd Azam Yusoff, Rosnina Abu Bakar @ Zakaria, Md Zuki Sarsaifi, Kazhal Modu, Bukar Muhammad Thein, Myint Kyaw, Than San, Maung Maung |
| author_facet | Naing, Soe Win Haron, Abd Wahid Goriman Khan, Mohd Azam Yusoff, Rosnina Abu Bakar @ Zakaria, Md Zuki Sarsaifi, Kazhal Modu, Bukar Muhammad Thein, Myint Kyaw, Than San, Maung Maung |
| author_sort | Naing, Soe Win |
| collection | UPM |
| description | In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (P < 0.05) sperm forward motility in post-thaw goat semen compared to trehalose or sucrose supplementation. Semen quality did not differ (P > 0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P < 0.05). Supplementation of trehalose (198.24 mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P < 0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24 mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation. |
| first_indexed | 2024-03-06T07:13:33Z |
| format | Article |
| id | upm.eprints-7888 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:13:33Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-78882016-11-16T07:43:58Z http://psasir.upm.edu.my/id/eprint/7888/ Effect of sugars on characteristics of Boer goat semen after cryopreservation Naing, Soe Win Haron, Abd Wahid Goriman Khan, Mohd Azam Yusoff, Rosnina Abu Bakar @ Zakaria, Md Zuki Sarsaifi, Kazhal Modu, Bukar Muhammad Thein, Myint Kyaw, Than San, Maung Maung In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (P < 0.05) sperm forward motility in post-thaw goat semen compared to trehalose or sucrose supplementation. Semen quality did not differ (P > 0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P < 0.05). Supplementation of trehalose (198.24 mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P < 0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24 mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation. Elsevier 2010-06-25 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7888/1/Effect%20of%20sugars%20on%20characteristics%20of%20Boer%20goat%20semen%20after%20cryopreservation.pdf Naing, Soe Win and Haron, Abd Wahid and Goriman Khan, Mohd Azam and Yusoff, Rosnina and Abu Bakar @ Zakaria, Md Zuki and Sarsaifi, Kazhal and Modu, Bukar Muhammad and Thein, Myint and Kyaw, Than and San, Maung Maung (2010) Effect of sugars on characteristics of Boer goat semen after cryopreservation. Animal Reproduction Science, 122 (1-2). pp. 23-28. ISSN 0378-4320; ESSN: 1873-2232 http://dx.doi.org/10.1016/j.anireprosci.2010.06.006 10.1016/j.anireprosci.2010.06.006 |
| spellingShingle | Naing, Soe Win Haron, Abd Wahid Goriman Khan, Mohd Azam Yusoff, Rosnina Abu Bakar @ Zakaria, Md Zuki Sarsaifi, Kazhal Modu, Bukar Muhammad Thein, Myint Kyaw, Than San, Maung Maung Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title | Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title_full | Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title_fullStr | Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title_full_unstemmed | Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title_short | Effect of sugars on characteristics of Boer goat semen after cryopreservation |
| title_sort | effect of sugars on characteristics of boer goat semen after cryopreservation |
| url | http://psasir.upm.edu.my/id/eprint/7888/1/Effect%20of%20sugars%20on%20characteristics%20of%20Boer%20goat%20semen%20after%20cryopreservation.pdf |
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