Rapid gene cloning in wheat
The identification of wheat and barley genes controlling important agronomic traits using positional cloning has traditionally been a challenging and time-consuming procedure. This is due to the enormous genome size and high repeat content from transposable elements (TEs). Low marker density, suppre...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Book Section |
| Language: | English |
| Published: |
Woodhead Publishing
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/78926/1/Rapid%20gene%20cloning%20in%20wheat.pdf |
| _version_ | 1796980531542360064 |
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| author | Md. Hatta, M. Asyraf Steuernagel, Burkhard Wulff, Brande B. H. |
| author2 | Miedaner, Thomas |
| author_facet | Miedaner, Thomas Md. Hatta, M. Asyraf Steuernagel, Burkhard Wulff, Brande B. H. |
| author_sort | Md. Hatta, M. Asyraf |
| collection | UPM |
| description | The identification of wheat and barley genes controlling important agronomic traits using positional cloning has traditionally been a challenging and time-consuming procedure. This is due to the enormous genome size and high repeat content from transposable elements (TEs). Low marker density, suppressed recombination, and the high cost of generating a physical contig across a genetically defined map interval have further restricted the application of positional approximation. Over the past decade, the cost of DNA sequencing has significantly dropped, as has our ability to computationally analyze large quantities of DNA sequence data. This has enabled researchers to exploit next-generation sequencing (NGS) technologies more routinely to accelerate the gene cloning process. In this chapter, we discuss several newly emerging cloning methods that combine NGS technologies with recent advances in molecular genomics to overcome previous limitations of gene cloning in wheat and barley |
| first_indexed | 2024-03-06T10:24:26Z |
| format | Book Section |
| id | upm.eprints-78926 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:24:26Z |
| publishDate | 2019 |
| publisher | Woodhead Publishing |
| record_format | dspace |
| spelling | upm.eprints-789262021-07-21T20:57:16Z http://psasir.upm.edu.my/id/eprint/78926/ Rapid gene cloning in wheat Md. Hatta, M. Asyraf Steuernagel, Burkhard Wulff, Brande B. H. The identification of wheat and barley genes controlling important agronomic traits using positional cloning has traditionally been a challenging and time-consuming procedure. This is due to the enormous genome size and high repeat content from transposable elements (TEs). Low marker density, suppressed recombination, and the high cost of generating a physical contig across a genetically defined map interval have further restricted the application of positional approximation. Over the past decade, the cost of DNA sequencing has significantly dropped, as has our ability to computationally analyze large quantities of DNA sequence data. This has enabled researchers to exploit next-generation sequencing (NGS) technologies more routinely to accelerate the gene cloning process. In this chapter, we discuss several newly emerging cloning methods that combine NGS technologies with recent advances in molecular genomics to overcome previous limitations of gene cloning in wheat and barley Woodhead Publishing Miedaner, Thomas Korzun, Viktor 2019 Book Section PeerReviewed text en http://psasir.upm.edu.my/id/eprint/78926/1/Rapid%20gene%20cloning%20in%20wheat.pdf Md. Hatta, M. Asyraf and Steuernagel, Burkhard and Wulff, Brande B. H. (2019) Rapid gene cloning in wheat. In: Applications of Genetic and Genomic Research in Cereals. Woodhead Publishing Series in Food Science, Technology and Nutrition . Woodhead Publishing, United Kingdom, 65 - 95. ISBN 9780081021637; EISBN: 9780081022139 https://www.sciencedirect.com/science/article/pii/B9780081021637000041#! 10.1016/B978-0-08-102163-7.00004-1 |
| spellingShingle | Md. Hatta, M. Asyraf Steuernagel, Burkhard Wulff, Brande B. H. Rapid gene cloning in wheat |
| title | Rapid gene cloning in wheat |
| title_full | Rapid gene cloning in wheat |
| title_fullStr | Rapid gene cloning in wheat |
| title_full_unstemmed | Rapid gene cloning in wheat |
| title_short | Rapid gene cloning in wheat |
| title_sort | rapid gene cloning in wheat |
| url | http://psasir.upm.edu.my/id/eprint/78926/1/Rapid%20gene%20cloning%20in%20wheat.pdf |
| work_keys_str_mv | AT mdhattamasyraf rapidgenecloninginwheat AT steuernagelburkhard rapidgenecloninginwheat AT wulffbrandebh rapidgenecloninginwheat |