Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)

An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. Th...

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Main Author: Mohd Amin, Khairuddin
Format: Project Paper Report
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf
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author Mohd Amin, Khairuddin
author_facet Mohd Amin, Khairuddin
author_sort Mohd Amin, Khairuddin
collection UPM
description An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. The bacteria were grown in minimal medium and sodium chloride. Through this assay, three distinct bacteria were isolated. Using the total ammonia-nitrogen (TAN) analysis, all three bacteria were able to degrade ammonium sulphate up to 0.04 ppm compared to the control of 0.54 ppm. All three bacteria were found to be Gram-positive bacteria by its distinctive purple color in Gram-staining method. Based on result, it can be concluded that the marine bacteria isolated from the culture water of Tiger grouper have the potential as bioremediator agent in aquaculture.
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spelling upm.eprints-792822020-07-07T00:38:46Z http://psasir.upm.edu.my/id/eprint/79282/ Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus) Mohd Amin, Khairuddin An experiment was conducted to isolate bacterial bioremediator from the culture water of Tiger Grouper (Epinephelus fuscoguttatus). In this study, the potential of the bacteria as ammonia degraders were investigated. This was done through enrichment assay using ammonium sulphate as carbon source. The bacteria were grown in minimal medium and sodium chloride. Through this assay, three distinct bacteria were isolated. Using the total ammonia-nitrogen (TAN) analysis, all three bacteria were able to degrade ammonium sulphate up to 0.04 ppm compared to the control of 0.54 ppm. All three bacteria were found to be Gram-positive bacteria by its distinctive purple color in Gram-staining method. Based on result, it can be concluded that the marine bacteria isolated from the culture water of Tiger grouper have the potential as bioremediator agent in aquaculture. 2012 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf Mohd Amin, Khairuddin (2012) Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus). [Project Paper Report]
spellingShingle Mohd Amin, Khairuddin
Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_full Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_fullStr Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_full_unstemmed Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_short Isolation of marine bacteria with bioremediation properties from the culture water of Tiger grouper (Epinephelus fuscoguttatus)
title_sort isolation of marine bacteria with bioremediation properties from the culture water of tiger grouper epinephelus fuscoguttatus
url http://psasir.upm.edu.my/id/eprint/79282/1/FP%202012%20103%20IR.pdf
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