Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus
Among different inbred chickens’ lines, we previously showed that lines P and N of Institute for Animal Health, Compton, UK are the most susceptible and the least affected lines, respectively, following infection with very virulent infectious bursal disease virus (vvIBDV). In this study, the differe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/79385/1/Differential%20expression%20of%20immune.pdf |
| _version_ | 1796980605295001600 |
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| author | Mohd Isa, Farhanah Abdullah Al-Haj, Nagi Ahmed Mat Isa, Nurulfiza Ideris, Aini Powers, Claire Oladapo, Omobolanle Nair, Venugopal Omar, Abdul Rahman |
| author_facet | Mohd Isa, Farhanah Abdullah Al-Haj, Nagi Ahmed Mat Isa, Nurulfiza Ideris, Aini Powers, Claire Oladapo, Omobolanle Nair, Venugopal Omar, Abdul Rahman |
| author_sort | Mohd Isa, Farhanah |
| collection | UPM |
| description | Among different inbred chickens’ lines, we previously showed that lines P and N of Institute for Animal Health, Compton, UK are the most susceptible and the least affected lines, respectively, following infection with very virulent infectious bursal disease virus (vvIBDV). In this study, the differential expressions of 29 different immune-related genes were characterized. Although, birds from both lines succumbed to infection, line P showed greater bursal lesion scores and higher viral copy numbers compared to line N. Interestingly, line N showed greater down-regulation of B cell related genes (BLNK, TNFSF13B and CD72) compared to line P. While up-regulation of T-cell related genes (CD86 and CTLA4) and Th1 associated cytokines (IFNG, IL2, IL12A and IL15) were documented in both lines, the expression levels of these genes were different in the two lines. Meanwhile, the expression of IFN-related genes IFNB, STAT1, and IRF10, but not IRF5, were up-regulated in both lines. The expression of pro-inflammatory cytokines (IL1B, IL6, IL18, and IL17) and chemokines (CXCLi2, CCL4, CCL5 and CCR5) were up-regulated in both lines with greater increase documented in line P compared to line N. Strikingly, the expression of IL12B was detected only in line P whilst the expression of IL15RA was detected only in line N. In conclusion, the bursal immunopathology of IBDV correlates more with expression of proinflammatory response related genes and does not related to expression of B-cell related genes. |
| first_indexed | 2024-03-06T10:25:29Z |
| format | Article |
| id | upm.eprints-79385 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:25:29Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-793852021-03-30T22:36:40Z http://psasir.upm.edu.my/id/eprint/79385/ Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus Mohd Isa, Farhanah Abdullah Al-Haj, Nagi Ahmed Mat Isa, Nurulfiza Ideris, Aini Powers, Claire Oladapo, Omobolanle Nair, Venugopal Omar, Abdul Rahman Among different inbred chickens’ lines, we previously showed that lines P and N of Institute for Animal Health, Compton, UK are the most susceptible and the least affected lines, respectively, following infection with very virulent infectious bursal disease virus (vvIBDV). In this study, the differential expressions of 29 different immune-related genes were characterized. Although, birds from both lines succumbed to infection, line P showed greater bursal lesion scores and higher viral copy numbers compared to line N. Interestingly, line N showed greater down-regulation of B cell related genes (BLNK, TNFSF13B and CD72) compared to line P. While up-regulation of T-cell related genes (CD86 and CTLA4) and Th1 associated cytokines (IFNG, IL2, IL12A and IL15) were documented in both lines, the expression levels of these genes were different in the two lines. Meanwhile, the expression of IFN-related genes IFNB, STAT1, and IRF10, but not IRF5, were up-regulated in both lines. The expression of pro-inflammatory cytokines (IL1B, IL6, IL18, and IL17) and chemokines (CXCLi2, CCL4, CCL5 and CCR5) were up-regulated in both lines with greater increase documented in line P compared to line N. Strikingly, the expression of IL12B was detected only in line P whilst the expression of IL15RA was detected only in line N. In conclusion, the bursal immunopathology of IBDV correlates more with expression of proinflammatory response related genes and does not related to expression of B-cell related genes. Elsevier 2020-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79385/1/Differential%20expression%20of%20immune.pdf Mohd Isa, Farhanah and Abdullah Al-Haj, Nagi Ahmed and Mat Isa, Nurulfiza and Ideris, Aini and Powers, Claire and Oladapo, Omobolanle and Nair, Venugopal and Omar, Abdul Rahman (2020) Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus. Comparative Immunology, Microbiology and Infectious Diseases, 68. ISSN 1878-1667; ESSN: 0147-9571 https://www.sciencedirect.com/science/article/abs/pii/S0147957119301961 10.1016/j.cimid.2019.101399 |
| spellingShingle | Mohd Isa, Farhanah Abdullah Al-Haj, Nagi Ahmed Mat Isa, Nurulfiza Ideris, Aini Powers, Claire Oladapo, Omobolanle Nair, Venugopal Omar, Abdul Rahman Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title | Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title_full | Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title_fullStr | Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title_full_unstemmed | Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title_short | Differential expression of immune-related genes in the bursa of Fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| title_sort | differential expression of immune related genes in the bursa of fabricius of two inbred chicken lines following infection with very virulent infectious bursal disease virus |
| url | http://psasir.upm.edu.my/id/eprint/79385/1/Differential%20expression%20of%20immune.pdf |
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