First nearly complete genome sequence of porcine bocavirus strain from Malaysia
This paper reports the first nearly complete genome sequence of a Malaysian Porcine bocavirus (PBoV) detected in Malaysia from a porcine organ sample. In Malaysia, both Human bocavirus (HBoV) and PBoV have been reported, at prevalence of 5.6% and 90.9% respectively. Most recently, in 2018, PBoV em...
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| Format: | Article |
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Nexus Academic Publishers
2019
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| _version_ | 1796980691049644032 |
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| author | Lee, Chee Yien Tan, Chew Yee Tan, Yi Jing Wong, Jeffrey Wai Nung Jacob, Daniel Mohan Lim, Jia Xin Ananthan, Gunaselvi Arshad, Siti Suri Selvarajah, Gayathri Thevi Ooi, Peck Toung |
| author_facet | Lee, Chee Yien Tan, Chew Yee Tan, Yi Jing Wong, Jeffrey Wai Nung Jacob, Daniel Mohan Lim, Jia Xin Ananthan, Gunaselvi Arshad, Siti Suri Selvarajah, Gayathri Thevi Ooi, Peck Toung |
| author_sort | Lee, Chee Yien |
| collection | UPM |
| description | This paper reports the first nearly complete genome sequence of a Malaysian Porcine bocavirus (PBoV)
detected in Malaysia from a porcine organ sample. In Malaysia, both Human bocavirus (HBoV) and PBoV have been
reported, at prevalence of 5.6% and 90.9% respectively. Most recently, in 2018, PBoV emerged as a zoonosis when a
human case of PBoV-related respiratory infection is reported in a child with history of contact with porcine secretion.
With this evidence of human infection with PBoV, considering close contact between humans and pigs in the swine
industry, further study of PBoV genetic characterization and epidemiology is warranted. This study attempts to obtain
the complete genome sequence of a Malaysian PBoV isolate detected in a pig farm in the state of Selangor. A nearly
complete sequence was obtained through primer walking and touchdown PCR method. Based on phylogenetic analysis for the nearly complete genome, the Malaysian PBoV strain is genetically characterized as PBoV G3B, sharing a
close relationship with a reference PBoV strain from U.S.A. with 98% homology. This finding may facilitate further
epidemiological studies and diagnostic development of PBoV in Malaysia. |
| first_indexed | 2024-03-06T10:26:46Z |
| format | Article |
| id | upm.eprints-79905 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:26:46Z |
| publishDate | 2019 |
| publisher | Nexus Academic Publishers |
| record_format | dspace |
| spelling | upm.eprints-799052023-03-23T05:09:58Z http://psasir.upm.edu.my/id/eprint/79905/ First nearly complete genome sequence of porcine bocavirus strain from Malaysia Lee, Chee Yien Tan, Chew Yee Tan, Yi Jing Wong, Jeffrey Wai Nung Jacob, Daniel Mohan Lim, Jia Xin Ananthan, Gunaselvi Arshad, Siti Suri Selvarajah, Gayathri Thevi Ooi, Peck Toung This paper reports the first nearly complete genome sequence of a Malaysian Porcine bocavirus (PBoV) detected in Malaysia from a porcine organ sample. In Malaysia, both Human bocavirus (HBoV) and PBoV have been reported, at prevalence of 5.6% and 90.9% respectively. Most recently, in 2018, PBoV emerged as a zoonosis when a human case of PBoV-related respiratory infection is reported in a child with history of contact with porcine secretion. With this evidence of human infection with PBoV, considering close contact between humans and pigs in the swine industry, further study of PBoV genetic characterization and epidemiology is warranted. This study attempts to obtain the complete genome sequence of a Malaysian PBoV isolate detected in a pig farm in the state of Selangor. A nearly complete sequence was obtained through primer walking and touchdown PCR method. Based on phylogenetic analysis for the nearly complete genome, the Malaysian PBoV strain is genetically characterized as PBoV G3B, sharing a close relationship with a reference PBoV strain from U.S.A. with 98% homology. This finding may facilitate further epidemiological studies and diagnostic development of PBoV in Malaysia. Nexus Academic Publishers 2019 Article PeerReviewed Lee, Chee Yien and Tan, Chew Yee and Tan, Yi Jing and Wong, Jeffrey Wai Nung and Jacob, Daniel Mohan and Lim, Jia Xin and Ananthan, Gunaselvi and Arshad, Siti Suri and Selvarajah, Gayathri Thevi and Ooi, Peck Toung (2019) First nearly complete genome sequence of porcine bocavirus strain from Malaysia. Advances in Animal and Veterinary Sciences, 7 (9). pp. 776-781. ISSN 2309-3331; ESSN: 2307-8316 http://nexusacademicpublishers.com/table_contents_detail/4/1209 10.17582/journal.aavs/2019/7.9.776.781 |
| spellingShingle | Lee, Chee Yien Tan, Chew Yee Tan, Yi Jing Wong, Jeffrey Wai Nung Jacob, Daniel Mohan Lim, Jia Xin Ananthan, Gunaselvi Arshad, Siti Suri Selvarajah, Gayathri Thevi Ooi, Peck Toung First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title | First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title_full | First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title_fullStr | First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title_full_unstemmed | First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title_short | First nearly complete genome sequence of porcine bocavirus strain from Malaysia |
| title_sort | first nearly complete genome sequence of porcine bocavirus strain from malaysia |
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