Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21
Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune su...
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| Format: | Article |
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Cell Press
2019
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| _version_ | 1796980707473489920 |
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| author | Abels, Erik R. Maas, Sybren L. N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L. D. Breakefield, Xandra O. |
| author_facet | Abels, Erik R. Maas, Sybren L. N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L. D. Breakefield, Xandra O. |
| author_sort | Abels, Erik R. |
| collection | UPM |
| description | Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression. |
| first_indexed | 2024-03-06T10:27:01Z |
| format | Article |
| id | upm.eprints-79998 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T10:27:01Z |
| publishDate | 2019 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | upm.eprints-799982023-05-30T08:42:51Z http://psasir.upm.edu.my/id/eprint/79998/ Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 Abels, Erik R. Maas, Sybren L. N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L. D. Breakefield, Xandra O. Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression. Cell Press 2019 Article PeerReviewed Abels, Erik R. and Maas, Sybren L. N. and Nieland, Lisa and Wei, Zhiyun and Cheah, Pike See and Tai, Eric and Kolsteeg, Christy-Joy and Dusoswa, Sophie A. and Ting, David T. and Hickman, Suzanne and El Khoury, Joseph and Krichevsky, Anna M. and Broekman, Marike L. D. and Breakefield, Xandra O. (2019) Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21. Cell Reports, 28 (12). pp. 3105-3119. ISSN 2211-1247 https://www.sciencedirect.com/science/article/pii/S2211124719310769?via%3Dihub 10.1016/j.celrep.2019.08.036 |
| spellingShingle | Abels, Erik R. Maas, Sybren L. N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L. D. Breakefield, Xandra O. Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title | Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title_full | Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title_fullStr | Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title_full_unstemmed | Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title_short | Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21 |
| title_sort | glioblastoma associated microglia reprogramming is mediated by functional transfer of extracellular mir 21 |
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