Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls
Growth Hormone (GH) is a single polypeptide chain synthesised and secreted from anterior pituitary gland by somatroph cells. The product of GH gene hastens metabolism and promotes the growth of many organs and tissues especially bone, muscle and visceral organs. It also regulates growth, mammary gla...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciencedomain International
2019
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/80131/1/Identification%20of%20bovine%20growth%20hormone%20%28BGH%29%20gene%20polymorphism%20using%20PCR-RFLP%20method%20in%20buffalo%20bulls.pdf |
| _version_ | 1796980730624999424 |
|---|---|
| author | Hazlan Shah, M. I. S. Salisi, Mohd Shahrom Haron, Abd Wahid Yahaya, M. S. Y., Rosnina Saad, Mohd Zamri Abu Hassim, Hasliza Omar, Mohamed Ariff Abdullah, Punimin Ahmad, Hafandi Md Isa, Nur Mahiza |
| author_facet | Hazlan Shah, M. I. S. Salisi, Mohd Shahrom Haron, Abd Wahid Yahaya, M. S. Y., Rosnina Saad, Mohd Zamri Abu Hassim, Hasliza Omar, Mohamed Ariff Abdullah, Punimin Ahmad, Hafandi Md Isa, Nur Mahiza |
| author_sort | Hazlan Shah, M. I. S. |
| collection | UPM |
| description | Growth Hormone (GH) is a single polypeptide chain synthesised and secreted from anterior pituitary gland by somatroph cells. The product of GH gene hastens metabolism and promotes the growth of many organs and tissues especially bone, muscle and visceral organs. It also regulates growth, mammary gland development and lactation. Polymorphism in this gene is associated with increase in growth and development of many tissues in the body. Aim: The objective of this study was to investigate the polymorphism of bovine growth hormone (bGH) gene in buffalo bulls (Bubalus bubalis) using the PCR-RFLP (polymerase chain reaction–restriction fragment length polymorphism) technique. Design: Genomic DNA was extracted from a total of 10 bulls, consisting of Murrah – Swamp crossbred and pure Swamp buffalo bulls. A The 446 segment of the bGH gene was amplified. The DNA amplicons were detected in 2% agarose gel following 45 minutes of electrophoresis. They were thereafter digesting with AluI endonuclease restriction enzyme, and the digested DNA were detected in 2% agarose gel following electrophoresis for about 45minutes in all samples Results: Similar bands of approximately 300 and 146-bp each, with no variation, were detected in 2% agarose gel following electrophoresis in all the animals tested. Conclusion: Based on the Alu1 digestion result, all samples produced the same allele of the gene, with no polymorphism detected. |
| first_indexed | 2024-03-06T10:27:21Z |
| format | Article |
| id | upm.eprints-80131 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:27:21Z |
| publishDate | 2019 |
| publisher | Sciencedomain International |
| record_format | dspace |
| spelling | upm.eprints-801312020-09-23T07:43:20Z http://psasir.upm.edu.my/id/eprint/80131/ Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls Hazlan Shah, M. I. S. Salisi, Mohd Shahrom Haron, Abd Wahid Yahaya, M. S. Y., Rosnina Saad, Mohd Zamri Abu Hassim, Hasliza Omar, Mohamed Ariff Abdullah, Punimin Ahmad, Hafandi Md Isa, Nur Mahiza Growth Hormone (GH) is a single polypeptide chain synthesised and secreted from anterior pituitary gland by somatroph cells. The product of GH gene hastens metabolism and promotes the growth of many organs and tissues especially bone, muscle and visceral organs. It also regulates growth, mammary gland development and lactation. Polymorphism in this gene is associated with increase in growth and development of many tissues in the body. Aim: The objective of this study was to investigate the polymorphism of bovine growth hormone (bGH) gene in buffalo bulls (Bubalus bubalis) using the PCR-RFLP (polymerase chain reaction–restriction fragment length polymorphism) technique. Design: Genomic DNA was extracted from a total of 10 bulls, consisting of Murrah – Swamp crossbred and pure Swamp buffalo bulls. A The 446 segment of the bGH gene was amplified. The DNA amplicons were detected in 2% agarose gel following 45 minutes of electrophoresis. They were thereafter digesting with AluI endonuclease restriction enzyme, and the digested DNA were detected in 2% agarose gel following electrophoresis for about 45minutes in all samples Results: Similar bands of approximately 300 and 146-bp each, with no variation, were detected in 2% agarose gel following electrophoresis in all the animals tested. Conclusion: Based on the Alu1 digestion result, all samples produced the same allele of the gene, with no polymorphism detected. Sciencedomain International 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80131/1/Identification%20of%20bovine%20growth%20hormone%20%28BGH%29%20gene%20polymorphism%20using%20PCR-RFLP%20method%20in%20buffalo%20bulls.pdf Hazlan Shah, M. I. S. and Salisi, Mohd Shahrom and Haron, Abd Wahid and Yahaya, M. S. and Y., Rosnina and Saad, Mohd Zamri and Abu Hassim, Hasliza and Omar, Mohamed Ariff and Abdullah, Punimin and Ahmad, Hafandi and Md Isa, Nur Mahiza (2019) Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls. Annual Research & Review in Biology (ARRB), 33 (1). pp. 1-8. ISSN 2347-565X 10.9734/ARRB/2019/v33i130111 |
| spellingShingle | Hazlan Shah, M. I. S. Salisi, Mohd Shahrom Haron, Abd Wahid Yahaya, M. S. Y., Rosnina Saad, Mohd Zamri Abu Hassim, Hasliza Omar, Mohamed Ariff Abdullah, Punimin Ahmad, Hafandi Md Isa, Nur Mahiza Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title | Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title_full | Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title_fullStr | Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title_full_unstemmed | Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title_short | Identification of bovine growth hormone (BGH) gene polymorphism using PCR-RFLP method in buffalo bulls |
| title_sort | identification of bovine growth hormone bgh gene polymorphism using pcr rflp method in buffalo bulls |
| url | http://psasir.upm.edu.my/id/eprint/80131/1/Identification%20of%20bovine%20growth%20hormone%20%28BGH%29%20gene%20polymorphism%20using%20PCR-RFLP%20method%20in%20buffalo%20bulls.pdf |
| work_keys_str_mv | AT hazlanshahmis identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT salisimohdshahrom identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT haronabdwahid identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT yahayams identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT yrosnina identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT saadmohdzamri identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT abuhassimhasliza identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT omarmohamedariff identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT abdullahpunimin identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT ahmadhafandi identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls AT mdisanurmahiza identificationofbovinegrowthhormonebghgenepolymorphismusingpcrrflpmethodinbuffalobulls |