Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α
Epigallocatechin-3-gallate (EGCG) is the most abundant bioactive polyphenolic compound among the green tea constituents and has been identified as a potential anticancer agent in colorectal cancer (CRC) studies. This study was aimed to determine the mechanism of actions of EGCG when targeting the en...
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| Format: | Article |
| Language: | English |
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Hindawi
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80246/1/Induction%20of%20endoplasmic%20reticulum%20stress%20pathway%20by%20green%20tea.pdf |
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| author | Md Nesran, Zarith Nameyrra Shafie, Nurul Husna Ishak, Amirah Haziyah Mohd Esa, Norhaizan Ismail, Amin Md Tohid, Siti Farah |
| author_facet | Md Nesran, Zarith Nameyrra Shafie, Nurul Husna Ishak, Amirah Haziyah Mohd Esa, Norhaizan Ismail, Amin Md Tohid, Siti Farah |
| author_sort | Md Nesran, Zarith Nameyrra |
| collection | UPM |
| description | Epigallocatechin-3-gallate (EGCG) is the most abundant bioactive polyphenolic compound among the green tea constituents and has been identified as a potential anticancer agent in colorectal cancer (CRC) studies. This study was aimed to determine the mechanism of actions of EGCG when targeting the endoplasmic reticulum (ER) stress pathway in CRC. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed on HT-29 cell line and normal cell line (3T3) to determine the EGCG toxicity. Next, western blot was done to observe the expression of the related proteins for the ER stress pathway. The Caspase 3/7 assay was performed to determine the apoptosis induced by EGCG. The results demonstrated that EGCG treatment was toxic to the HT-29 cell line. EGCG induced ER stress in HT-29 by upregulating immunoglobulin-binding (BiP), PKR-like endoplasmic reticulum kinase (PERK), phosphorylation of eukaryotic initiation factor 2 alpha subunit (eIF2α), activating transcription 4 (ATF4), and inositol-requiring kinase 1 alpha (IRE1α). Apoptosis was induced in HT-29 cells after the EGCG treatment, as shown by the Caspase 3/7 activity. This study indicates that green tea EGCG has the potential to inhibit colorectal cancer cells through the induction of ER stress. |
| first_indexed | 2024-03-06T10:27:39Z |
| format | Article |
| id | upm.eprints-80246 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:27:39Z |
| publishDate | 2019 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | upm.eprints-802462020-10-19T17:02:33Z http://psasir.upm.edu.my/id/eprint/80246/ Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α Md Nesran, Zarith Nameyrra Shafie, Nurul Husna Ishak, Amirah Haziyah Mohd Esa, Norhaizan Ismail, Amin Md Tohid, Siti Farah Epigallocatechin-3-gallate (EGCG) is the most abundant bioactive polyphenolic compound among the green tea constituents and has been identified as a potential anticancer agent in colorectal cancer (CRC) studies. This study was aimed to determine the mechanism of actions of EGCG when targeting the endoplasmic reticulum (ER) stress pathway in CRC. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed on HT-29 cell line and normal cell line (3T3) to determine the EGCG toxicity. Next, western blot was done to observe the expression of the related proteins for the ER stress pathway. The Caspase 3/7 assay was performed to determine the apoptosis induced by EGCG. The results demonstrated that EGCG treatment was toxic to the HT-29 cell line. EGCG induced ER stress in HT-29 by upregulating immunoglobulin-binding (BiP), PKR-like endoplasmic reticulum kinase (PERK), phosphorylation of eukaryotic initiation factor 2 alpha subunit (eIF2α), activating transcription 4 (ATF4), and inositol-requiring kinase 1 alpha (IRE1α). Apoptosis was induced in HT-29 cells after the EGCG treatment, as shown by the Caspase 3/7 activity. This study indicates that green tea EGCG has the potential to inhibit colorectal cancer cells through the induction of ER stress. Hindawi 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80246/1/Induction%20of%20endoplasmic%20reticulum%20stress%20pathway%20by%20green%20tea.pdf Md Nesran, Zarith Nameyrra and Shafie, Nurul Husna and Ishak, Amirah Haziyah and Mohd Esa, Norhaizan and Ismail, Amin and Md Tohid, Siti Farah (2019) Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α. Biomed Research International, 2019. art. no. 3480569. pp. 1-9. ISSN 2314-6141; ESSN: 2314-6133 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942794/ 10.1155/2019/3480569 |
| spellingShingle | Md Nesran, Zarith Nameyrra Shafie, Nurul Husna Ishak, Amirah Haziyah Mohd Esa, Norhaizan Ismail, Amin Md Tohid, Siti Farah Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title | Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title_full | Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title_fullStr | Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title_full_unstemmed | Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title_short | Induction of endoplasmic reticulum stress pathway by green tea epigallocatechin-3-gallate (EGCG) in colorectal cancer cells: activation of PERK/p-eIF2α/ATF4 and IRE1α |
| title_sort | induction of endoplasmic reticulum stress pathway by green tea epigallocatechin 3 gallate egcg in colorectal cancer cells activation of perk p eif2α atf4 and ire1α |
| url | http://psasir.upm.edu.my/id/eprint/80246/1/Induction%20of%20endoplasmic%20reticulum%20stress%20pathway%20by%20green%20tea.pdf |
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