Intestinal permeability and transport of apigenin across caco-2 cell monolayers
Investigation on bioavailability was carried out by determining the absorption and transport of bioaccessible apigenin from Mangifera indica (Water Lily var.) into Caco-2 human intestinal cell using a reliable and sensitive analytical method of LC-MS/MS. Results revealed that the concentration of gl...
| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
International Society for Nutraceuticals and Functional Foods
2019
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| Acesso em linha: | http://psasir.upm.edu.my/id/eprint/80311/1/Intestinal%20permeability%20and%20transport%20of%20apigenin%20across%20caco-2%20cell%20monolayers.pdf |
| _version_ | 1825951295904677888 |
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| author | Zulkifli, Marina Ismail, Amin Loh, Su Peng Jailani, Fadhilah Kassim, Nur Kartinee |
| author_facet | Zulkifli, Marina Ismail, Amin Loh, Su Peng Jailani, Fadhilah Kassim, Nur Kartinee |
| author_sort | Zulkifli, Marina |
| collection | UPM |
| description | Investigation on bioavailability was carried out by determining the absorption and transport of bioaccessible apigenin from Mangifera indica (Water Lily var.) into Caco-2 human intestinal cell using a reliable and sensitive analytical method of LC-MS/MS. Results revealed that the concentration of glucuronidated apigenin lower than apigenin. The apigenin was metabolised inside the cells through glucuronidation process, and cross the monolayer to reach the basolateral sides or effluxed back to the apical side. The permeability coefficient of apigenin from apical to basolateral sides and basolateral to apical sides showed a medium permeability was less than 20 x10-6 cm.sec-1. Since the value of efflux ratio was 1.5, it suggested that the apigenin was absorbed and transported through a simple diffusion mechanism. |
| first_indexed | 2024-03-06T10:27:50Z |
| format | Article |
| id | upm.eprints-80311 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:27:50Z |
| publishDate | 2019 |
| publisher | International Society for Nutraceuticals and Functional Foods |
| record_format | dspace |
| spelling | upm.eprints-803112020-10-21T18:30:26Z http://psasir.upm.edu.my/id/eprint/80311/ Intestinal permeability and transport of apigenin across caco-2 cell monolayers Zulkifli, Marina Ismail, Amin Loh, Su Peng Jailani, Fadhilah Kassim, Nur Kartinee Investigation on bioavailability was carried out by determining the absorption and transport of bioaccessible apigenin from Mangifera indica (Water Lily var.) into Caco-2 human intestinal cell using a reliable and sensitive analytical method of LC-MS/MS. Results revealed that the concentration of glucuronidated apigenin lower than apigenin. The apigenin was metabolised inside the cells through glucuronidation process, and cross the monolayer to reach the basolateral sides or effluxed back to the apical side. The permeability coefficient of apigenin from apical to basolateral sides and basolateral to apical sides showed a medium permeability was less than 20 x10-6 cm.sec-1. Since the value of efflux ratio was 1.5, it suggested that the apigenin was absorbed and transported through a simple diffusion mechanism. International Society for Nutraceuticals and Functional Foods 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80311/1/Intestinal%20permeability%20and%20transport%20of%20apigenin%20across%20caco-2%20cell%20monolayers.pdf Zulkifli, Marina and Ismail, Amin and Loh, Su Peng and Jailani, Fadhilah and Kassim, Nur Kartinee (2019) Intestinal permeability and transport of apigenin across caco-2 cell monolayers. Journal of Food Bioactives, 7. pp. 48-55. ISSN 2637-8752; ESSN: 2637-8779 http://www.isnff-jfb.com/index.php/JFB/article/view/108 10.31665/JFB.2019.7198 |
| spellingShingle | Zulkifli, Marina Ismail, Amin Loh, Su Peng Jailani, Fadhilah Kassim, Nur Kartinee Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title | Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title_full | Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title_fullStr | Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title_full_unstemmed | Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title_short | Intestinal permeability and transport of apigenin across caco-2 cell monolayers |
| title_sort | intestinal permeability and transport of apigenin across caco 2 cell monolayers |
| url | http://psasir.upm.edu.my/id/eprint/80311/1/Intestinal%20permeability%20and%20transport%20of%20apigenin%20across%20caco-2%20cell%20monolayers.pdf |
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